Anti-CDC42 antibody [28-10] (ab255897)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Mouse monoclonal [28-10] to CDC42
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-CDC42 antibody [28-10]
See all CDC42 primary antibodies -
Description
Mouse monoclonal [28-10] to CDC42 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman -
Immunogen
Recombinant full length protein within CDC42. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P19073 -
Positive control
- WB: Jurkat, THP1, PC-12 and NIH/3T3 whole cell lysate. Mouse and rat brain and spleen tissue lysate.
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
28-10 -
Isotype
IgG2b -
Research areas
Images
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All lanes : Anti-CDC42 antibody [28-10] (ab255897) at 0.544 µg/ml
Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 2 : THP1 (human monocytic leukemia monocyte), whole cell lysate
Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 21 kDaBlocking/Dilution buffer: 5% NFDM/TBST.
Exposure times: Lane 1: 5 seconds; Lane 2-4: 26 seconds.
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Lanes 1 & 3 : Anti-CDC42 antibody [28-10] (ab255897) at 0.544 µg/ml
Lanes 2 & 4 : Anti-CDC42 antibody [28-10] (ab255897) at 0.554 µg/ml
Lane 1 : Mouse brain tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat brain tissue lysate
Lane 4 : Rat spleen tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution
Predicted band size: 21 kDa
Exposure time: 3 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
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