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Anti-CDC42 antibody [28-10] (ab255897)

Price and availability

307 566 ₸

Availability

Order now and get it on Tuesday March 16, 2021

Anti-CDC42 antibody [28-10] (ab255897)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Mouse monoclonal [28-10] to CDC42
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human

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Overview

  • Product name

    Anti-CDC42 antibody [28-10]
    See all CDC42 primary antibodies
  • Description

    Mouse monoclonal [28-10] to CDC42
  • Host species

    Mouse
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Rat
    Human
    See all applications and species data
  • Immunogen

    Recombinant full length protein within CDC42. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
    Database link: P19073

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • WB: Jurkat, THP1, PC-12 and NIH/3T3 whole cell lysate. Mouse and rat brain and spleen tissue lysate.
  • General notes

    This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    28-10
  • Isotype

    IgG2b
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Cytokinesis
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Other
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Images

  • Western blot - Anti-CDC42 antibody [28-10] (ab255897)
    Western blot - Anti-CDC42 antibody [28-10] (ab255897)
    All lanes : Anti-CDC42 antibody [28-10] (ab255897) at 0.544 µg/ml

    Lane 1 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
    Lane 2 : THP1 (human monocytic leukemia monocyte), whole cell lysate
    Lane 3 : NIH/3T3 (mouse embryonic fibroblast), whole cell lysate
    Lane 4 : PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 21 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure times: Lane 1: 5 seconds; Lane 2-4: 26 seconds.

  • Western blot - Anti-CDC42 antibody [28-10] (ab255897)
    Western blot - Anti-CDC42 antibody [28-10] (ab255897)
    Lanes 1 & 3 : Anti-CDC42 antibody [28-10] (ab255897) at 0.544 µg/ml
    Lanes 2 & 4 : Anti-CDC42 antibody [28-10] (ab255897) at 0.554 µg/ml

    Lane 1 : Mouse brain tissue lysate
    Lane 2 : Mouse spleen tissue lysate
    Lane 3 : Rat brain tissue lysate
    Lane 4 : Rat spleen tissue lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Peroxidase-Conjugated Goat anti-Mouse IgG (H+L) at 1/5000 dilution

    Predicted band size: 21 kDa


    Exposure time: 3 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-CDC42 antibody [28-10] (ab255897)
    Anti-CDC42 antibody [28-10] (ab255897)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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