Anti-TDG antibody (ab227498)
Key features and details
- Rabbit polyclonal to TDG
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TDG antibody
See all TDG primary antibodies -
Description
Rabbit polyclonal to TDG -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanWB Human
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Immunogen
Recombinant fragment within Human TDG (internal sequence). The exact sequence is proprietary.
Database link: Q13569 -
Positive control
- WB: HeLa cell extract; Jurakt, Raji and NCI-H929 whole cell extracts. ICC/IF: HeLa cells.
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General notes
Previously labelled as Thymine DNA glycosylase.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 79.99% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TDG antibody (ab227498)All lanes : Anti-TDG antibody (ab227498) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : TDG Knockout HeLa cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa
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Western blot - Anti-TDG antibody (ab227498)All lanes : Anti-TDG antibody (ab227498) at 1/500 dilution
Lane 1 : Wild-type HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lane 2 : TDG knockout HeLa (human epithelial cell line from cervix adenocarcinoma) cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa7.5% SDS-PAGE gel.
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Western blot - Anti-TDG antibody (ab227498)All lanes : Anti-TDG antibody (ab227498) at 1/500 dilution
Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
Lane 3 : NCI-H929 whole cell extract
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 46 kDa10% SDS-PAGE gel.
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Immunocytochemistry/ Immunofluorescence - Anti-TDG antibody (ab227498)4% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for TDG (green) using ab227498 at 1/1000 dilution in ICC/IF.
Blue: Hoechst 33342 staining.
