Anti-TDG antibody (ab106301)
Key features and details
- Rabbit polyclonal to TDG
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-TDG antibody
See all TDG primary antibodies -
Description
Rabbit polyclonal to TDG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Chicken, Chinese hamster
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Immunogen
Synthetic peptide corresponding to Human TDG aa 250-350 conjugated to keyhole limpet haemocyanin.
(Peptide available asab124618) -
Positive control
- Recombinant Human TDG protein (ab114876) can be used as a positive control in WB. This antibody gave a positive signal in the following whole cell lysates: HeLa; Jurkat; HEK293; A431; Hues7.
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General notes
Previously labelled as Thymine DNA glycosylase.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab106301 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 46 kDa). IP Use a concentration of 5 µg/ml. Target
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Function
In the DNA of higher eukaryotes, hydrolytic deamination of 5-methylcytosine to thymine leads to the formation of G/T mismatches. This enzyme corrects G/T mispairs to G/C pairs. It is capable of hydrolyzing the carbon-nitrogen bond between the sugar-phosphate backbone of the DNA and a mispaired thymine. In addition to the G/T, it can remove thymine also from C/T and T/T mispairs in the order G/T >> C/T > T/T. It has no detectable activity on apyrimidinic sites and does not catalyze the removal of thymine from A/T pairs or from single-stranded DNA. It can also remove uracil and 5-bromouracil from mispairs with guanine. -
Sequence similarities
Belongs to the TDG/mug DNA glycosylase family. -
Post-translational
modificationsSumoylation on Lys-330 by either SUMO1 or SUMO2 induces dissociation of the product DNA. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6996 Human
- Entrez Gene: 21665 Mouse
- Entrez Gene: 114521 Rat
- Omim: 601423 Human
- SwissProt: Q13569 Human
- SwissProt: P56581 Mouse
- Unigene: 584809 Human
- Unigene: 284252 Mouse
see all -
Alternative names
- C JUN leucine zipper interactive protein antibody
- C JUN leucine zipper interactive protein antibody
- C-JUN leucine zipper interactive protein JZA-3 antibody
see all
Images
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Immunoprecipitation - Anti-TDG antibody (ab106301)
TDG was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to TDG and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab106301.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 50kDa; TDG
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Western blot - Anti-TDG antibody (ab106301)All lanes : Anti-TDG antibody (ab106301) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes -
Western blot - Anti-TDG antibody (ab106301)This image is courtesy of an anonymous AbreviewAll lanes : Anti-TDG antibody (ab106301) at 1 µg/ml
All lanes : Mouse ES whole cell lysate
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Protocols
Datasheets and documents
References (1)
ab106301 has been referenced in 1 publication.
- Zhou L et al. GADD45a Promotes Active DNA Demethylation of the MMP-9 Promoter via Base Excision Repair Pathway in AGEs-Treated Keratinocytes and in Diabetic Male Rat Skin. Endocrinology 159:1172-1186 (2018). WB ; Human . PubMed: 29244109
Images
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Immunoprecipitation - Anti-TDG antibody (ab106301)
TDG was immunoprecipitated using 0.5mg A431 whole cell extract, 5µg of Rabbit polyclonal to TDG and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, A431 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab106301.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 50kDa; TDG
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Western blot - Anti-TDG antibody (ab106301)All lanes : Anti-TDG antibody (ab106301) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 5 : HUES7 (Human embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 22 kDa, 78 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 2 minutes
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Western blot - Anti-TDG antibody (ab106301) This image is courtesy of an anonymous AbreviewAll lanes : Anti-TDG antibody (ab106301) at 1 µg/ml
All lanes : Mouse ES whole cell lysate
Lysates/proteins at 100 µg per lane.
Secondary
All lanes : HRP Goat anti-rabbit IgG polyclonal at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 46 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
