Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸ 10 µl 134 016 ₸

Order now and get it on Tuesday March 02, 2021

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR8774] to TDG
Suitable for: Flow Cyt, WB
Reacts with: Mouse, Rat, Human

Product name

Anti-TDG antibody [EPR8774]
See all TDG primary antibodies
Description

Rabbit monoclonal [EPR8774] to TDG
Host species

Rabbit
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Mouse

Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to Human TDG.
Database link: Q13569
Positive control
- Fetal lung tissue, Jurkat cell lysate, HeLa cells and HeLa cell lysate, K562 cell lysate and Fetal brain lysate
General notes
Previously labelled as Thymine DNA glycosylase.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Mouse Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR8774
Isotype

IgG
Research areas

Western blot - Anti-TDG antibody [EPR8774] (ab154192)

All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/5000 dilution

Lane 1 : Mouse brain tissue at 20 µg
Lane 2 : mouse heart tissue at 20 µg
Lane 3 : mouse kidney tissue
Lane 4 : mouse spleen tissue at 20 µg
Lane 5 : rat brain tissue at 20 µg
Lane 6 : rat heart tissue at 20 µg
Lane 7 : rat kidney tissue at 20 µg
Lane 8 : rat spleen tissue at 20 µg

Predicted band size: 46 kDa
Observed band size: 55-60 kDa
why is the actual band size different from the predicted?

Exposure time: 3 minutes
Western blot - Anti-TDG antibody [EPR8774] (ab154192)

All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/1000 dilution

Lane 1 : Fetal lung cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lane 5 : Fetal brain cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 46 kDa
Flow Cytometry - Anti-TDG antibody [EPR8774] (ab154192)

Flow cytometric analysis of permeabilized HeLa cells labeling TDG with ab154192 at 1/10 dilution (red) compared to a negative control rabbit IgG antibody (green).
Anti-TDG antibody [EPR8774] (ab154192)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com