Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TDG antibody [EPR8774] (ab154192)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR8774] to TDG
  • Suitable for: Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TDG antibody [EPR8774]
    See all TDG primary antibodies

  • Description

    Rabbit monoclonal [EPR8774] to TDG

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide corresponding to Human TDG.
    Database link: Q13569

  • Positive control

    • Fetal lung tissue, Jurkat cell lysate, HeLa cells and HeLa cell lysate, K562 cell lysate and Fetal brain lysate

  • General notes

    Previously labelled as Thymine DNA glycosylase.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...

  • Purity

    Tissue culture supernatant

  • Clonality

    Monoclonal

  • Clone number

    EPR8774

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-TDG antibody [EPR8774] (ab154192)
    Western blot - Anti-TDG antibody [EPR8774] (ab154192)
    All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/5000 dilution

    Lane 1 : Mouse brain tissue at 20 µg
    Lane 2 : mouse heart tissue at 20 µg
    Lane 3 : mouse kidney tissue
    Lane 4 : mouse spleen tissue at 20 µg
    Lane 5 : rat brain tissue at 20 µg
    Lane 6 : rat heart tissue at 20 µg
    Lane 7 : rat kidney tissue at 20 µg
    Lane 8 : rat spleen tissue at 20 µg

    Predicted band size: 46 kDa
    Observed band size: 55-60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 3 minutes
  • Western blot - Anti-TDG antibody [EPR8774] (ab154192)
    Western blot - Anti-TDG antibody [EPR8774] (ab154192)
    All lanes : Anti-TDG antibody [EPR8774] (ab154192) at 1/1000 dilution

    Lane 1 : Fetal lung cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : K562 cell lysate
    Lane 5 : Fetal brain cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 46 kDa

  • Flow Cytometry - Anti-TDG antibody [EPR8774] (ab154192)
    Flow Cytometry - Anti-TDG antibody [EPR8774] (ab154192)

    Flow cytometric analysis of permeabilized HeLa cells labeling TDG with ab154192 at 1/10 dilution (red) compared to a negative control rabbit IgG antibody (green).

  • Anti-TDG antibody [EPR8774] (ab154192)
    Anti-TDG antibody [EPR8774] (ab154192)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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