Anti-Spermine antibody (ab26975)
Key features and details
- Rabbit polyclonal to Spermine
- Suitable for: ICC/IF, WB
- Reacts with: Species independent
- Isotype: IgG
Overview
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Product name
Anti-Spermine antibody -
Description
Rabbit polyclonal to Spermine -
Host species
Rabbit -
Specificity
This antibody reacts with spermine. Cross-reacts with spermidine 100%. -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Spermine conjugated to KLH.
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General notes
Polyamines are substrates for transglutaminase enzymes and can be covalently conjugated to proteins. In this case Western Blot and other methods can be able to detect polyaminated proteins.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading... -
Purity
Whole antiserum -
Primary antibody notes
Polyamines are substrates for transglutaminase enzymes and can be covalently conjugated to proteins. In this case Western Blot and other methods can be able to detect polyaminated proteins. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Spermine antibody (ab26975) This image is courtesy of an anonymous Abreviewab26975 staining spermine in the Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with 4% formaldehyde for 15 minuts at room temperature (Upper images) or iced methanol of 3 minutes (Lower images) and blocked with 2% BSA for 30 minutes at 27°C. Samples were incubated with primary antibody (1/100) for 1 hour at 27°C. An Alexa Fluor®568-conjugated Donkey anti-rabbit polyclonal (1/1000) was used as the secondary antibody. Samples handled without ab26975 were used as negative controls (Left-hand panels).