Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Wednesday March 10, 2021

Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [E180] to Caspase-6/CASP-6
Suitable for: WB, IHC-P, ICC/IF
Reacts with: Human

Product name

Anti-Caspase-6/CASP-6 antibody [E180]
See all Caspase-6/CASP-6 primary antibodies
Description

Rabbit monoclonal [E180] to Caspase-6/CASP-6
Host species

Rabbit
Specificity

This antibody detects the pro form and the p11 subunit of human Caspase-6/CASP-6. We have data to indicate that this antibody may not cross react with Rat. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.

Tested Applications & Species

Application	Species
ICC/IF	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide within Human Caspase-6/CASP-6 aa 250-350 (C terminal). The exact sequence is proprietary.
Database link: P55212
Positive control
- WB: Jurkat cell lysate. ICC/IF: Hela cells. IHC-P: Human lung carcinoma tissue slides.
General notes
This product was previously labelled as Caspase-6.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Clonality

Monoclonal
Clone number

E180
Isotype

IgG
Research areas

Western blot - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)

All lanes : Anti-Caspase-6/CASP-6 antibody [E180] (ab32366) at 1/1000 dilution

Lane 1 : Untreated Jurkat cell lysate.

Lane 2 : Jurkat cell lysate treated with Camptothecin.

Predicted band size: 34 kDa
Observed band size: 30 kDa
why is the actual band size different from the predicted?
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)

Ab32366, at a 1:50 dilution, staining Caspase-6/CASP-6 in paraffin embedded human lung adenocarcinoma tissue by Immunohistochemistry.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)

Ab32366, at a 1:100 dilution, staining Caspase-6/CASP-6 in Hela cells by Immunocytochemistry/Immunofluorescence.
Anti-Caspase-6/CASP-6 antibody [E180] (ab32366)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com