Antibodies, Proteins, Kits and Reagents for Life Science

274 732 ₸ Product size

100 µl 274 732 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6
Suitable for: WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-Caspase-6/CASP-6 antibody [EPR4405]
See all Caspase-6/CASP-6 primary antibodies
Description

Rabbit monoclonal [EPR4405] to Caspase-6/CASP-6
Host species

Rabbit
Specificity

ab108335 detects both the full length and cleaved forms of Caspase-6/CASP-6.
Tested Applications & Species

Application Species
Flow Cyt
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Jurkat cells and cell lysate.
General notes
This product was previously labelled as Caspase-6.

This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
Flow Cyt	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR4405
Isotype

IgG
Research areas

Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)

All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : CASP6 knockout HAP1 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 33 kDa

Lanes 1 - 2: Merged signal (red and green). Green - ab108335 observed at 37 kDa. Red - loading control, ab7291, observed at 50 kDa.

ab108335 was shown to specifically react with in wild-type HAP1 cells as signal was lost in CASP6 knockout cells. Wild-type and CASP6 knockout samples were subjected to SDS-PAGE. The membrane was blocked with 3% NF Milk. Ab108335 and ab7291 (Mouse anti Tubulin loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)

All lanes : Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : Jurkat + staurosporine cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 33 kDa
Flow Cytometry - Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)

ab108335, at 1:10 dilution, staining Caspase-6/CASP-6 in permeabilized Jurkat cells by Flow cytometry (shown in red). Rabbit IgG shown in green (negative).
Anti-Caspase-6/CASP-6 antibody [EPR4405] (ab108335)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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