Anti-MEKK3 antibody [ep600y] (ab40756)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [ep600y] to MEKK3
- Suitable for: ICC/IF, IHC-P, WB, Flow Cyt, IP
- Reacts with: Human
Overview
-
Product name
Anti-MEKK3 antibody [ep600y]
See all MEKK3 primary antibodies -
Description
Rabbit monoclonal [ep600y] to MEKK3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIHC-P HumanWB HumanRecombinant fragment -
Immunogen
Synthetic peptide within Human MEKK3 aa 600-700 (C terminal). The exact sequence is proprietary.
-
Positive control
- HeLa cells, ovary carcinoma
-
General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
ep600y -
Isotype
IgG -
Research areas
Images
-
All lanes : Anti-MEKK3 antibody [ep600y] (ab40756) at 1/1000 dilution
Lane 1 :Recombinant human MEKK2 protein (ab135009)
Lane 2 :Recombinant human MEKK3 protein (ab85297)
Lysates/proteins at 0.015 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 71 kDa
Observed band size: 97 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking and diluting buffer: 5% NFDM/TBST
-
Immunofluorescent staining of HeLa cells using ab40756 diluted 1:100
-
Anti-MEKK3 antibody [ep600y] (ab40756) at 1/1000 dilution + HeLa cell lysate at 10 µg
Predicted band size: 71 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
-
Immunohistochemical analysis of paraffin-embedded human ovary carcinoma using ab40756 diluted 1:100
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
-