Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2863] to TAK1 (phospho S439) - BSA and Azide free
  • Suitable for: Dot blot, IP, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free
    See all TAK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR2863] to TAK1 (phospho S439) - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Dot blot, IP, WBmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.

  • General notes

    ab239974 is the carrier-free version of ab109404. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239974 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
    Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)

    Purified ab109404 at 1/50 dilution (2µg) immunoprecipitating TAK1 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
    Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate 10µg
    Lane 2 (+): ab109404 + HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab239974 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 75 kDa
    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109404).

  • Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
    Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)

    Dot Blot analysis of Lane 1: TAK1 (phospho S439) phospho peptide and Lane 2: TAK1 non-phospho peptide labeling TAK1 (phospho S439) with ab109404 at 1/1000 dilution (0.009 μg/ml). 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109404).

  • Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)
    Anti-TAK1 (phospho S439) antibody [EPR2863] - BSA and Azide free (ab239974)

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