Antibodies, Proteins, Kits and Reagents for Life Science

307 566 ₸ Product size

100 µl 307 566 ₸ 10 µl 103 862 ₸

Order now and get it on Tuesday March 23, 2021

Anti-TAK1 antibody [EPR22903-57] (ab239353)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR22903-57] to TAK1
Suitable for: WB
Reacts with: Drosophila melanogaster

Product name

Anti-TAK1 antibody [EPR22903-57]
See all TAK1 primary antibodies
Description

Rabbit monoclonal [EPR22903-57] to TAK1
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Drosophila melanogaster

See all applications and species data
Immunogen

Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild type Drosophila and Drosophila Tak1[2] mutant (with a point mutation resulting in a 53aa truncated protein) lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

Learn more here.

Application	Species
WB	Drosophila melanogaster

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR22903-57
Isotype

IgG
Research areas

Western blot - Anti-TAK1 antibody [EPR22903-57] (ab239353)

Anti-TAK1 antibody [EPR22903-57] (ab239353) at 1/1000 dilution + Drosophila schneider 2 (S2) cell whole cell lysate at 10 µl

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 76 kDa
Observed band size: 85 kDa
why is the actual band size different from the predicted?

Exposure time: 26 seconds

Blocking/Diluting buffer: 5% NFDM/TBST
Western blot - Anti-TAK1 antibody [EPR22903-57] (ab239353)

All lanes : Anti-TAK1 antibody [EPR22903-57] (ab239353) at 1/1000 dilution

Lane 1 : Wild type Drosophila lysate
Lane 2 : Drosophila Tak1[2] mutant (with a point mutation resulting in a 53aa truncated protein) lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

Predicted band size: 76 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

This blot was developed using a higher sensitivity ECL substrate.
The lysates were kindly provided by an anonymous collaborator.

Exposure time: 3 minutes
Anti-TAK1 antibody [EPR22903-57] (ab239353)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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