Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)
![Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)](https://www.abcam.com/ps/products/270/ab270758/Images/ab270758-396111-anti-tak1-antibody-epr22903-57-western-blot-drosophila.jpg "Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22903-57] to TAK1 - BSA and Azide free
- Suitable for: WB
- Reacts with: Drosophila melanogaster
Overview
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Product name
Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free
See all TAK1 primary antibodies -
Description
Rabbit monoclonal [EPR22903-57] to TAK1 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Drosophila melanogaster
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Wild type Drosophila and Drosophila Tak1[2] mutant (with a point mutation resulting in a 53aa truncated protein) lysates.
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General notes
ab270758 is the carrier-free version of ab239353. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22903-57 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)Anti-TAK1 antibody [EPR22903-57] (ab239353) at 1/1000 dilution + Drosophila schneider 2 (S2) cell whole cell lysate at 10 µl
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 76 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?
Exposure time: 26 secondsBlocking/Diluting buffer: 5% NFDM/TBST
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab239353).
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![Western blot - Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)](https://www.abcam.com/ps/products/270/ab270758/Images/ab270758-383506-ab239353383503ANTITA1.jpg)
Western blot - Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)All lanes : Anti-TAK1 antibody [EPR22903-57] (ab239353) at 1/1000 dilution
Lane 1 : Wild type Drosophila lysate
Lane 2 : Drosophila Tak1[2] mutant (with a point mutation resulting in a 53aa truncated protein) lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 76 kDa
Observed band size: 85 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST.
This blot was developed using a higher sensitivity ECL substrate.
The lysates were kindly provided by an anonymous collaborator.Exposure time: 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab239353).
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![Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)](https://www.abcam.com/ps/products/270/ab270758/Images/ab270758-7-benefits-of-recombinant-antibodies.png)
Anti-TAK1 antibody [EPR22903-57] - BSA and Azide free (ab270758)
