Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Thursday March 04, 2021

Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2863] to TAK1 (phospho S439)
Suitable for: Dot blot, WB, IP
Reacts with: Human

Product name

Anti-TAK1 (phospho S439) antibody [EPR2863]
See all TAK1 primary antibodies
Description

Rabbit monoclonal [EPR2863] to TAK1 (phospho S439)
Host species

Rabbit
Specificity

ab109404 only detects TAK1 phosphorylated at Serine 439 in Human or TAK1 phosphorylated at Serine 412 in Mouse.
Tested Applications & Species

Application Species
IP
Human

WB
Human

See all applications and species data
Immunogen

Phospho specific peptide against residues surrounding Serine 439 of Human TAK1.
Positive control
- HeLa cell lysate. IP: HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IP	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR2863
Isotype

IgG
Research areas

Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

All lanes : Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

Lane 1 : HeLa whole cell lysate
Lane 2 : HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1ß for 10 minutes whole cell lysate
Lane 3 : HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1ß for 10 minutes whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 67 kDa
Observed band size: 75 kDa
why is the actual band size different from the predicted?

Exposure time: 10 seconds

Dilution and blocking buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

Purified ab109404 at 1/50 dilution (2µg) immunoprecipitating TAK1 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.

Lane 1 (input): HeLa (Human cervix adenocarcinoma epithelial cell) treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate 10µg
Lane 2 (+): ab109404 + HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab239974 in HeLa treated with 100nM Calyculin A and 20ng/ml human IL-1 beta for 10min whole cell lysate.

VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 75 kDa
Dot Blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

Dot Blot analysis of Lane 1: TAK1 (phospho S439) phospho peptide and Lane 2: TAK1 non-phospho peptide labeling TAK1 (phospho S439) with ab109404 at 1/1000 dilution (0.009 μg/ml). 5% NFDM/TBST was used as the diluting and blocking buffer. ab97051 Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated was used as the secondary antibody at 1/100,000 dilution. Exposure time: 3 minutes.
Western blot - Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

All lanes : Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HeLa cell lysate treated with transforming growth factor-beta (TGF-beta)

Lysates/proteins at 10 µg per lane.

Predicted band size: 67 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Anti-TAK1 (phospho S439) antibody [EPR2863] (ab109404)

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