Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸

Order now and get it on Tuesday March 09, 2021

Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [Y83] to Rsk 2 / MAPKAP Kinase 1b
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Human

Product name

Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83]
See all Rsk 2 / MAPKAP Kinase 1b primary antibodies
Description

Rabbit monoclonal [Y83] to Rsk 2 / MAPKAP Kinase 1b
Host species

Rabbit
Specificity

The antibody recognises Rsk2. It does not cross react with other S6 kinase family members.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

A synthetic peptide corresponding to residues near N-terminus of human RSK2
Positive control
- HeLa whole cell lysate (ab150035) and human lymphoma
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 49% PBS, 50% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

IgG fraction
Clonality

Monoclonal
Clone number

Y83
Isotype

IgG
Research areas

Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)

Lane 1: Wild-type HAP1 cell lysate (40 µg)
Lane 2: Rsk 2 / MAPKAP Kinase 1b knockout HAP1 cell lysate (40 µg)
Lane 3: HeLa cell lysate (40 µg)
Lane 4: (40 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab32133 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab32133 was shown to specifically react with Rsk 2 / MAPKAP Kinase 1b when Rsk 2 / MAPKAP Kinase 1b knockout samples were used. Wild-type and Rsk 2 / MAPKAP Kinase 1b knockout samples were subjected to SDS-PAGE. Ab32133 and ab8245 (loading control to GAPDH) were diluted at 1/5000 and 1:10,000 dilution respectively and incubated overnight at 4C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed ab216773
andGoat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed ab216776
secondary antibodies at 1:10,000 dilution for 1 hour at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)

ab32133, staining human lymphoma by immunohistochemistry, Paraffin embedded tissue

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Western blot - Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)

Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133) at 1/5000 dilution + Hela cell lysate

Predicted band size: 84 kDa
Observed band size: 90 kDa
why is the actual band size different from the predicted?
Anti-Rsk 2 / MAPKAP Kinase 1b antibody [Y83] (ab32133)

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