Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR12698] to Wnt5a - C-terminal
  • Suitable for: ICC/IF, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-Wnt5a antibody [EPR12698] - C-terminal
    See all Wnt5a primary antibodies

  • Description

    Rabbit monoclonal [EPR12698] to Wnt5a - C-terminal

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Wnt5a aa 300 to the C-terminus. The exact sequence is proprietary.
    Database link: P41221

  • Positive control

    • Human melanoma lysates, Human breast cancer lysate ; HeLa cells

  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Properties

Images

  • Western blot - Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)
    Western blot - Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)
    All lanes : Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824) at 1/1000 dilution

    Lane 1 : Human breast cancer lysate
    Lane 2 : Human melanoma lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 42 kDa



    Blocking/Diluting buffer 5% NFDM/TBST

    Exposure time: Lane 1: 3 minutes 
    Lane 2: 1 minute

     

  • Immunocytochemistry/ Immunofluorescence - Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)
    Immunocytochemistry/ Immunofluorescence - Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)

    Immunofluorescent staining of HeLa cells labeling Wnt5a using ab179824 at 1/50 dilution (green). DAPI nuclear staining (blue).

  • Flow Cytometry - Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)
    Flow Cytometry - Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)

    Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling Wnt5a with purified ab179824 at 1/20 dilution (10ug/mL) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

  • Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)
    Anti-Wnt5a antibody [EPR12698] - C-terminal (ab179824)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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