Anti-Wnt5a antibody (ab229200)
Key features and details
- Rabbit polyclonal to Wnt5a
- Suitable for: IHC-P, IHC-Fr, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Wnt5a antibody
See all Wnt5a primary antibodies -
Description
Rabbit polyclonal to Wnt5a -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Xenopus laevis
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Immunogen
Synthetic peptide corresponding to Human Wnt5a aa 300 to the C-terminus conjugated to keyhole limpet haemocyanin.
Database link: P41221 -
Positive control
- WB: Mouse ovary and uterus tissue lysates. IHC-P: Human lung carcinoma tissue. IHC-Fr: Mouse brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 1% BSA, 50% Glycerol (glycerin, glycerine)
Aqueous buffered solution -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Wnt5a antibody (ab229200)
Formalin-fixed, paraffin-embedded human lung carcinoma tissue stained for Wnt5a using ab229200 at 1/200 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody and DAB staining.
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Western blot - Anti-Wnt5a antibody (ab229200)Anti-Wnt5a antibody (ab229200) at 1/300 dilution + Mouse ovary tissue lysate
Secondary
Conjugated secondary antibody at 1/10000 dilution
Predicted band size: 42 kDa
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Immunohistochemistry (Frozen sections) - Anti-Wnt5a antibody (ab229200)Frozen mouse brain tissue stained for Wnt5a using ab229200 at 1/1000 dilution in immunohistochemical analysis, followed by conjugation to the secondary antibody.
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Western blot - Anti-Wnt5a antibody (ab229200)Anti-Wnt5a antibody (ab229200) at 1/300 dilution + Mouse uterus tissue lysate
Secondary
Conjugated secondary antibody at 1/10000 dilution
Predicted band size: 42 kDa
