Anti-SEMA3B antibody (ab88489)
Key features and details
- Rabbit polyclonal to SEMA3B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-SEMA3B antibody
See all SEMA3B primary antibodies -
Description
Rabbit polyclonal to SEMA3B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide corresponding to Human SEMA3B aa 100-200 conjugated to keyhole limpet haemocyanin.
(Peptide available asab101720) -
Positive control
- This antibody gave a positive signal in Human brain tissue lysate.
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General notes
This product was previously labelled as Semaphorin 3B
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88489 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
GuaranteedTested applications are guaranteed to work and covered by our Abpromise guarantee.
PredictedPredicted to work for this combination of applications and species but not guaranteed.
IncompatibleDoes not work for this combination of applications and species.
Application Species WB HumanAll applications MouseRatApplication Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa).Notes WB
Use a concentration of 1 µg/ml. Detects a band of approximately 50 kDa (predicted molecular weight: 83 kDa).Target
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Function
Inhibits axonal extension by providing local signals to specify territories inaccessible for growing axons. -
Tissue specificity
Expressed abundantly but differentially in a variety of neural and nonneural tissues. -
Sequence similarities
Belongs to the semaphorin family.
Contains 1 Ig-like C2-type (immunoglobulin-like) domain.
Contains 1 PSI domain.
Contains 1 Sema domain. -
Cellular localization
Secreted. Endoplasmic reticulum. Accumulates in the endoplasmic reticulum. - Information by UniProt
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Database links
- Entrez Gene: 7869 Human
- Entrez Gene: 20347 Mouse
- Omim: 601281 Human
- SwissProt: Q13214 Human
- SwissProt: Q0VGP2 Mouse
- SwissProt: Q62177 Mouse
- Unigene: 82222 Human
- Unigene: 4083 Mouse
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Alternative names
- SEM3B_HUMAN antibody
- Sema A(V) antibody
- Sema V antibody
see all
Images
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Western blot - Anti-SEMA3B antibody (ab88489)Anti-SEMA3B antibody (ab88489) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThe band observed at 50 kDa represents the secreted form of SEMA3B.
Datasheets and documents
References (0)
ab88489 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-SEMA3B antibody (ab88489)Anti-SEMA3B antibody (ab88489) at 1 µg/ml + Human brain tissue lysate - total protein (ab29466) at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 83 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 20 kDa, 36 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutesThe band observed at 50 kDa represents the secreted form of SEMA3B.
