Antibodies, Proteins, Kits and Reagents for Life Science

348 441 ₸ Product size

100 µl 348 441 ₸ 10 µl 137 366 ₸

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Anti-DCAMKL1 antibody [EPR6085] (ab109029)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR6085] to DCAMKL1
Suitable for: WB, IHC-P
Knockout validated
Reacts with: Mouse, Human

Product name

Anti-DCAMKL1 antibody [EPR6085]
See all DCAMKL1 primary antibodies
Description

Rabbit monoclonal [EPR6085] to DCAMKL1
Host species

Rabbit
Specificity

The mouse and rat recommendation is for WB use only. We do not guarantee IHC-P for mouse and rat.
Tested Applications & Species

Application Species
IHC-P
Human

WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- Human fetal lung, NIH3T3, and SH-SY5Y lysates, Human brain tissue
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
IHC-P	Human
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR6085
Isotype

IgG
Research areas

Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)

Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
Lane 2: Knockout HAP1 whole cell lysate (20 µg)
Lane 3: DCLK1 (KO) whole cell lysate (20 µg)
Lane 4: Empty whole cell lysate (20 µg)
Lane 5: Hu brain whole cell lysate (20 µg)
Lanes 1 - 5: Merged signal (red and green). Green - ab109029 observed at 90 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab109029 was shown to specifically react with DCLK1 in wild-type HAP1 cells along with additional cross reactive bands. No bands were observed when knockout samples were used. Wild-type and knockout samples were subjected to SDS-PAGE. Ab109029 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-DCAMKL1 antibody [EPR6085] (ab109029)

All lanes : Anti-DCAMKL1 antibody [EPR6085] (ab109029) at 1/1000 dilution

Lane 1 : Human fetal lung lysate
Lane 2 : NIH3T3 lysate
Lane 3 : SH-SY5Y lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 82 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCAMKL1 antibody [EPR6085] (ab109029)

Immunohistochemical analysis of paraffin-embedded Human brain tissue using ab109029 at a dilution of 1/100.

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Anti-DCAMKL1 antibody [EPR6085] (ab109029)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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