Antibodies, Proteins, Kits and Reagents for Life Science

284 784 ₸ Product size

100 µl 284 784 ₸ 10 µl 113 243 ₸

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Anti-MDH2 antibody [EPR14883(B)] (ab181857)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14883(B)] to MDH2
Suitable for: WB, IHC-P, Flow Cyt
Knockout validated
Reacts with: Human

Product name

Anti-MDH2 antibody [EPR14883(B)]
See all MDH2 primary antibodies
Description

Rabbit monoclonal [EPR14883(B)] to MDH2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Human

See all applications and species data

Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HEK293T, K562, HepG2, and HeLa whole cell lysates; Human eyeball tissue lysate. IHC-P: Human hepatocellular carcinoma tissue Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Tissue culture supernatant
Clonality

Monoclonal
Clone number

EPR14883(B)
Isotype

IgG
Research areas

Western blot - Anti-MDH2 antibody [EPR14883(B)] (ab181857)

All lanes : Anti-MDH2 antibody [EPR14883(B)] (ab181857) at 1/1000 dilution

Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : MDH2 knockout HEK293T cell lysate
Lane 3 : K-562 cell lysate
Lane 4 : Human eyeball tissue lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa

Lanes 1-4: Merged signal (red and green). Green - ab181857 observed at 36 kDa. Red - loading control ab7291 observed at 50 kDa.

ab181857 Anti-MDH2 antibody [EPR14883(B)] was shown to specifically react with MDH2 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266449 (knockout cell lysate ab257533) was used. Wild-type and MDH2 knockout samples were subjected to SDS-PAGE. ab181857 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Flow Cytometry - Anti-MDH2 antibody [EPR14883(B)] (ab181857)

Flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling MDH2 with ab181857 at 1/10 dilution (red) compared to a Rabbit IgG monoclonal isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody [EPR14883(B)] (ab181857)

Immunohistochemical analysis of paraffin-embedded Human hepatocellular carcinoma tissue labeling MDH2 with ab181857 at 1/50 dilution, followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.
Western blot - Anti-MDH2 antibody [EPR14883(B)] (ab181857)

All lanes : Anti-MDH2 antibody [EPR14883(B)] (ab181857) at 1/50000 dilution

Lane 1 : K562 cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 36 kDa
Observed band size: 36 kDa
Anti-MDH2 antibody [EPR14883(B)] (ab181857)

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