Anti-MDH2 antibody (ab96193)
Key features and details
- Rabbit polyclonal to MDH2
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-MDH2 antibody
See all MDH2 primary antibodies -
Description
Rabbit polyclonal to MDH2 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Zebrafish
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Immunogen
Recombinant fragment corresponding to Human MDH2 aa 40-289.
Database link: P40926 -
Positive control
- 293T , A431 , HeLa , HepG2 , mouse brain and heart, rat lung.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MDH2 antibody (ab96193)All lanes :
Lane 1 : Wild-type HeLa whole cell lysate
Lane 2 : MDH2 knockout HeLa whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE
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Immunocytochemistry/ Immunofluorescence - Anti-MDH2 antibody (ab96193)Immunofluorescence staining of ice-cold methanol-fixed (5 minutes) HeLa cells stained for MDH2 with ab96193 at 1:500 dilution. Blue: Hoechst 33342 staining.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (ab96193)Immunohistochemical analysis of paraffin embedded mouse heart tissue labelling MDH2 with ab96193 antibody at 1/500 dilution at the mitochondria.
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Western blot - Anti-MDH2 antibody (ab96193)All lanes : Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : HepG2 whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MDH2 antibody (ab96193)Immunohistochemical analysis of paraffin embedded H1299 xenograft tissue labelling MDH2 with ab96193 antibody at 1/500 dilution.
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Western blot - Anti-MDH2 antibody (ab96193)Mouse brain at 20 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS PAGE
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Western blot - Anti-MDH2 antibody (ab96193)Rat lung at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG
Predicted band size: 36 kDa10% SDS-PAGE
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Western blot - Anti-MDH2 antibody (ab96193) This image is courtesy of an Anonymous Abreview.All lanes : Anti-MDH2 antibody (ab96193) at 1/1000 dilution
Lane 1 : Mouse C2C12 whole cell lysate
Lane 2 : Mouse C2C12 whole cell lysate on day 1 of differentiation
Lane 3 : Mouse C2C12 whole cell lysate on day 2 of differentiation
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : HRP-conjugated Goat anti-Rabbit IgG at 1/100000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 36 kDa
Observed band size: 46 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
