Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17848-87] to CYFIP2 + CYFIP1 - BSA and Azide free
  • Suitable for: Flow Cyt, WB, IHC-P, ICC
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free
    See all CYFIP2 + CYFIP1 primary antibodies

  • Description

    Rabbit monoclonal [EPR17848-87] to CYFIP2 + CYFIP1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, WB, IHC-P, ICCmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • General notes

    Ab251411 is the carrier-free version of ab204129. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251411 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C. Do Not Freeze.

  • Storage buffer

    pH: 7.2
    Constituent: PBS

  • Carrier free

    Yes
  • Concentration information loading...

  • Clonality

    Monoclonal

  • Clone number

    EPR17848-87

  • Isotype

    IgG

Images

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/50000 dilution + Human CYFIP1 Recombinant protein fragment at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa
    Observed band size: 148 kDa


    Exposure time: 10 seconds


    This data was developed using ab204129, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    All lanes : Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/5000 dilution

    Lane 1 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate at 20 µg
    Lane 2 : Molt4 (Human lymphoblastic leukemia cell line) whole cell lysate at 20 µg
    Lane 3 : Human fetal kidney lysate at 10 µg
    Lane 4 : Human fetal brain lysate at 10 µg

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa
    Observed band size: 148 kDa


    Exposure time: 1 second


    This data was developed using ab204129, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    All lanes : Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/5000 dilution

    Lane 1 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 2 : SW480 (Human colon adenocarcinoma cell line) whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa
    Observed band size: 148 kDa


    Exposure time: 15 seconds


    This data was developed using ab204129, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Western blot - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] (ab204129) at 1/1000 dilution + RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

    Predicted band size: 148 kDa
    Observed band size: 146 kDa
    why is the actual band size different from the predicted?


    Exposure time: 1 second


    This data was developed using ab204129, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    This data was developed using ab204129, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human tonsil labeling CYFIP2 + CYFIP1 with ab204129 at 1/300 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasmic staining on human tonsil tissue is observed. Counter stained with Hematoxylin. Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
  • Immunocytochemistry - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Immunocytochemistry - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    This data was developed using ab204129, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% Methanol, 0.1% Triton X-100 permeabilized Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CYFIP2 + CYFIP1 with ab204129 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on JurKat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab204129 at 1/150 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Immunocytochemistry - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Immunocytochemistry - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    This data was developed using ab204129, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 100% Methanol, 0.1% Triton X-100 permeabilized SW480 (Human colon adenocarcinoma cell line) cells labeling CYFIP2 + CYFIP1 with ab204129 at 1/150 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on SW480 cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).
    The negative controls are as follows:
    1. ab204129 at 1/150 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
    2. ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.
  • Flow Cytometry - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Flow Cytometry - Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    This data was developed using ab204129, the same antibody clone in a different buffer formulation.Flow cytometric analysis of 4% paraformaldehyde-fixed Jurkat (Human T cell leukemia cells from peripheral blood) cells labeling CYFIP2 + CYFIP1 with ab204129 at 1/150 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/500 dilution was used as the secondary antibody.
  • Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)
    Anti-CYFIP2 + CYFIP1 antibody [EPR17848-87] - BSA and Azide free (ab251411)

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