Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19725] to Ornithine Carbamoyltransferase/OTC - BSA and Azide free
  • Suitable for: IHC-Fr, WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free
    See all Ornithine Carbamoyltransferase/OTC primary antibodies

  • Description

    Rabbit monoclonal [EPR19725] to Ornithine Carbamoyltransferase/OTC - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-Fr, WB, IHC-P, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: Human fetal liver and small intestine lysates; Mouse and rat liver lysates. IHC-P: Human liver and liver cancer tissues. IHC-Fr: Mouse liver tissue. IP: Human fetal liver lysate.

  • General notes

    ab222399 is the carrier-free version of ab203859 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab222399 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Previously labelled as Ornithine Carbamoyltransferase.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)

    Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human liver tissue is observed [PMID: 17893704].

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)

    Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasmic staining on Human liver cancer tissue is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. 

    No staining on Human kidney is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab97051 at 1/500 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)
    Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse liver tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) at 1/1000 dilution (green).

    Cytoplasmic staining on mouse liver is observed.

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

  • Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)
    Immunohistochemistry (Frozen sections) - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized Mouse kidney tissue labeling Ornithine Carbamoyltransferase/OTC with ab203859 at 1/500 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077)  at 1/1000 dilution (green).

    Weakly staining on mouse kidney.

    Nuclear counterstain: DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ab150077 at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

  • Immunoprecipitation - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)
    Immunoprecipitation - Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)

    Ornithine Carbamoyltransferase/OTC Western blot was performed from the immunoprecipitate using ab203859 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Human fetal liver lysate, 10µg (Input).

    Lane 2: ab203859 IP in human fetal liver lysate.

    Lane 3: Rabbit IgG,monoclonal [EPR25A] - Isotype Control (ab172730) instead of ab203859 in human fetal liver lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 minutes.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab203859).

  • Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)
    Anti-Ornithine Carbamoyltransferase/OTC antibody [EPR19725] - BSA and Azide free (ab222399)

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