Recombinant Human NPHS2 protein (ab188448)
Key features and details
- Expression system: Escherichia coli
- Purity: > 95% Densitometry
- Endotoxin level:
- Tags: His tag N-Terminus
- Suitable for: SDS-PAGE, MS, WB, ELISA
Preparation and Storage
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Stability and Storage
Shipped at 4°C. Upon delivery aliquot. Store at -80°C. Avoid freeze / thaw cycle.
Constituents: 0.02% DTT, 0.25% Sodium acetate
Lyophilized in 30 mM acetate buffer. -
ReconstitutionAdd 0.1 M Acetate buffer pH4 to prepare a working stock solution of approximately 0.5 mg/mL and let the lyophilized pellet dissolve completely. For conversion into higher pH value, we recommend intensive dilution by relevant buffer to a concentration of 10 µg/mL. In higher concentrations the solubility of this antigen is limited.
Images
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SDS-PAGE - Recombinant Human NPHS2 protein (ab188448)
14% SDS-PAGE analysis of ab188448.
Lane 1: Reduced and boiled sample, 2.5 μg
Lane 2: Non-reduced and non-boiled sample, 2.5 μg