Myristoylated Protein Assay Kit (Green) (ab273290)
Key features and details
- Assay type: Cell-based (qualitative)
- Detection method: Fluorescent
- Platform: Flow cytometer, Fluorescence microscope
- Sample type: Adherent cells, Suspension cells
Overview
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Product name
Myristoylated Protein Assay Kit (Green)
See all Myristoylated Protein kits -
Detection method
Fluorescent -
Sample type
Adherent cells, Suspension cells -
Assay type
Cell-based (qualitative) -
Product overview
Myristoylated Protein Activity Assay Kit (Green) (ab273290) a highly specific, simple and robust method for labeling and detection of Myristoylated proteins. The kit uses Alkynyl Myristic Acid that is fed directly into the cells and gets incorporated into proteins during or post translation. This modification can be followed by click reaction with an azide-containing dye. The assay kit offers a powerful method for imaging localization, trafficking, and dynamics of Myristoylated proteins, or detection by FACS for quantitative studies. Labeled Myristoylated Proteins can be directly detected in 1D or 2D gels using the appropriate excitation sources or enriched by immunoprecipitation with biotin-azide or antibodies prior to proteomic analysis.
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Tested applications
Suitable for: FM, Flow Cytmore details -
Platform
Flow cytometer, Fluorescence microscope
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests Copper Reagent (100X) 1 x 100µl Fixative solution 1 x 10ml Fluorescent Azide (100X) 1 x 100µl Myristic Acid Label 1 x 10µl Permeabilization Buffer (10X) 1 x 25ml Reducing Agent (20X) 1 x 500µl Total DNA stain (1000X) 1 x 10µl Wash Buffer (10X) 1 x 25ml
Images
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Myristic Acid labeling of myristoylated protein in Jurkat cells
Metabolic labeling of Myristic Acid on proliferating Jurkat cells. 1 x 106 cells were incubated overnight with fresh aliquots of media containing Myristic Acid Label: Flow cytometry analysis of Negative Control (Black), Background (Fluorescent Azide only, Green), Positive Control (Myristic Acid Label and Fluorescent Azide, Red). Signal measured in FL-1 channel clearly shows the Myristic Aicd Labeling of Protein.
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Myristic Acid labeling of myristoylated protein in HeLa cellsMetabolic labeling of Myristic Acid on proliferating HeLa cells. 1 x 105 cells incubated overnight with fresh aliquots of media containing Myristic Acid Label: Upper panel corresponds to the Fluorescent Azide only Background fluorescence on HeLa cells. The lower panel shows that the red fluorescence corresponds to the Myristic Acid Label labeling reaction. Nuclear staining in both panels confirms that green signal is a result of Myristic Acid Label incorporation