Astana Biomed Group, an authorized Abcam distributor in Central Asia

Human GBP1 ELISA Kit (ab270897)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 24.09 pg/ml
  • Range: 109.38 pg/ml - 7000 pg/ml
  • Sample type: Cit plasma, Hep Plasma
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human GBP1 ELISA Kit
    See all GBP1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Plasma 8 8.3%
    Inter-assay
    Sample n Mean SD CV%
    Plasma 3 8.7%

  • Sample type

    Hep Plasma, Cit plasma

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    24.09 pg/ml

  • Range

    109.38 pg/ml - 7000 pg/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Hep Plasma 85 82% - 87%
    Cit plasma 99 91% - 104%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human GBP1 ELISA kit (ab270897) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of GBP1 protein in human citrate and heparin plasma. It uses our proprietary SimpleStep ELISA® technology. Quantitate human GBP1 with 24.09 ng/mL sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            -Single-wash protocol reduces assay time to 90 minutes or less
            -High sensitivity, specificity and reproducibility from superior antibodies
            -Fully validated in biological samples
            -96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Human Guanylate-binding protein 1 (GBP1) is a 67 kDa GTPase. Protein expression is induced by response to interferon gamma. GBP1 hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. It has been reported to have antiviral activity against the influenza virus. In addition, GBP1 has been shown to promote oxidative killing and delivery of antimicrobial peptides the autophagolysosomes, providing broad host protection against different pathogen classes. Human GBP1 shares 69%, 72%, 22% sequence identity with mouse, rat, and cow, respectively.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Human GBP1 Capture Antibody 1 x 600µl
    10X Human GBP1 Detector Antibody 1 x 600µl
    10X Wash Buffer PT (ab206977) 1 x 20ml
    Antibody Diluent CPI - HAMA Blocker (ab193969) 1 x 6ml
    Human GBP1 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS (ab193972) 1 x 12ml
    SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit
    Stop Solution 1 x 12ml
    TMB Development Solution 1 x 12ml

  • Research areas

  • Function

    Binds GTP, GDP and GMP.

  • Sequence similarities

    Belongs to the GBP family.

  • Cellular localization

    Cell membrane.
  • Information by UniProt

  • Alternative names

    • GBP 1
    • GBP-1
    • GBP1
    • GBP1_HUMAN
    • GTP binding protein 1
    • GTP-binding protein 1
    • Guanine nucleotide binding protein 1
    • Guanine nucleotide-binding protein 1
    • Guanylate binding protein 1
    • Guanylate binding protein 1 interferon inducible
    • Guanylate binding protein 1 interferon inducible 67kDa
    • HuGBP 1
    • HuGBP-1
    • HuGBP1
    • Interferon induced guanylate binding protein 1
    • Interferon-induced guanylate-binding protein 1
    • OTTHUMP00000012352
    see all

  • Database links

    Images

    • SimpleStep ELISA Protocol Diagram
      SimpleStep ELISA Protocol Diagram

      SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

    • Example of human GBP1 standard curve in Sample Diluent NS.
      Example of human GBP1 standard curve in Sample Diluent NS.

      The GBP1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

    • Interpolated concentrations of native GBP1 in human plasma samples.
      Interpolated concentrations of native GBP1 in human plasma samples.

      The concentrations of GBP1 were measured in duplicates, interpolated from the GBP1 standard curves and corrected for sample dilution. Undiluted samples are as follows: plasma (citrate) 50% and plasma (heparin) 100%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean GBP1 concentration was determined to be 1182.48 pg/mL in plasma (citrate) and 789.88 pg/mL in and plasma (heparin).

    • Sandwich ELISA - Human GBP1 ELISA Kit (ab270897)
      Sandwich ELISA - Human GBP1 ELISA Kit (ab270897)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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