DSCAM ELISA Kit (ab269543)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 38.39 pg/ml
- Range: 203.13 pg/ml - 13000 pg/ml
- Sample type: Cell Lysate, Tissue Extracts
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Mouse, Rat, Human
Overview
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Product name
DSCAM ELISA Kit
See all Dscam kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Extract 8 11% -
Sample type
Tissue Extracts, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
38.39 pg/ml -
Range
203.13 pg/ml - 13000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Tissue Extracts 101 % - % Cell Lysate 99 % - % -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
DSCAM ELISA Kit (ab269543) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of DSCAM protein in cell lysate and tissue extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate DSCAM with 38.39 pg/ml sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
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Notes
DSCAM is an adhesion protein expressed in the highest level in the fetal brain, although DSCAM is also expressed in other tissues and in adults. DSCAM is involved in neurogenesis and self-avoidance, but also in immunity in invertebrates. Overexpression of DSCAM in the fetal brain leads to Down syndrome, and altered DSCAM levels have been observed in PTSD, bipolar disorder, depression and Alzheimer disease. DSCAM has high amino acid sequence identity between human, mouse and rat. The standard protein in this product is human DSCAM.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests DSCAM Lyophilized Recombinant Protein 2 vials 10X DSCAM Capture Antibody 1 x 600µl 10X DSCAM Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 50X Cell Extraction Enhancer Solution (ab193971) 1 x 1ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent CPR 1 x 6ml Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
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Function
Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. -
Tissue specificity
Primarily expressed in brain. -
Sequence similarities
Contains 6 fibronectin type-III domains.
Contains 10 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Ig-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC. -
Post-translational
modificationsPhosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin. -
Cellular localization
Secreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons. - Information by UniProt
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Alternative names
- CHD2
- CHD2 42
- CHD2 52
see all -
Database links
- Entrez Gene: 1826 Human
- Entrez Gene: 13508 Mouse
- Entrez Gene: 171119 Rat
- Omim: 602523 Human
- SwissProt: O60469 Human
- SwissProt: Q9ERC8 Mouse
- SwissProt: Q8VHZ8 Rat
- Unigene: 397800 Human
Images
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SimpleStep ELISA Protocol Diagram
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
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Example of DSCAM standard curve in 1X Cell Extraction Buffer PTRThe DSCAM standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.
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Interpolated concentrations of native DSCAM in mouse and rat brain homogenate extract based on a 1,000 µg/mL extract load.The concentrations of DSCAM were measured in duplicate and interpolated from the DSCAM standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean DSCAM concentration was determined to be 1,792.70 pg/ml in 1,000 μg/mL mouse brain homogenate extract and 1,651.44 pg/ml in 1,000 μg/mL rat brain homogenate extract.
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Sandwich ELISA - DSCAM ELISA Kit (ab269543)To learn more about the advantages of recombinant antibodies see here.
