DSCAM Antibody Pair - BSA and Azide free (ab270380)
Key features and details
- Unconjugated capture and detector antibodies
- Adaptable to any antibody pair-based assay format
- Antibody concentration ~ 1 mg/ml
- BSA and azide free buffer - ready for conjugation
- Reacts with: Mouse, Rat, Human
Overview
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Product name
DSCAM Antibody Pair - BSA and Azide free
See all Dscam kits -
Assay type
ELISA set -
Range
203.125 pg/ml - 13000 pg/ml -
Species reactivity
Reacts with: Mouse, Rat, Human -
Product overview
The Antibody Pair can be used to quantify DSCAM. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.
For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab269543), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.
Download SDS here.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Carrier free
Yes -
Components 10 x 96 tests DSCAM Capture Antibody (unconjugated) 1 x 100µg DSCAM Detector Antibody (unconjugated) 1 x 100µg -
Research areas
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Function
Cell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38. -
Tissue specificity
Primarily expressed in brain. -
Sequence similarities
Contains 6 fibronectin type-III domains.
Contains 10 Ig-like C2-type (immunoglobulin-like) domains. -
Domain
Ig-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC. -
Post-translational
modificationsPhosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin. -
Cellular localization
Secreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons. - Information by UniProt
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Alternative names
- CHD2
- CHD2 42
- CHD2 52
see all -
Database links
- Entrez Gene: 1826 Human
- Entrez Gene: 13508 Mouse
- Entrez Gene: 171119 Rat
- Omim: 602523 Human
- SwissProt: O60469 Human
- SwissProt: Q9ERC8 Mouse
- SwissProt: Q8VHZ8 Rat
- Unigene: 397800 Human
see all
Images
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Sandwich ELISA - DSCAM Antibody Pair - BSA and Azide free (ab270380)To learn more about the advantages of recombinant antibodies see here.
