Anti-Cdc25B antibody [EPR3459(2)] (ab124819)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3459(2)] to Cdc25B
- Suitable for: WB, IP
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Cdc25B antibody [EPR3459(2)]
See all Cdc25B primary antibodies -
Description
Rabbit monoclonal [EPR3459(2)] to Cdc25B -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman -
Immunogen
Synthetic peptide within Human Cdc25B aa 150-250. The exact sequence is proprietary.
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Positive control
- K562 cell lysate, U937 cell lysate and THP-1 cell lysate, mouse embryo E17 and NIH3T3 cell lysates
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General notes
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3459(2) -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 0.486 µg/ml
Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) cell lysates
Lane 2 : Mouse embryo E17 lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml
Predicted band size: 64 kDa
Observed band size: 64 kDa
Blocking/diluting Buffer and concentration: 5% NFDM /TBST -
All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : U937 cell lysate
Lane 3 : THP-1 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat-anti-rabbit HRP at 1/2000 dilution
Predicted band size: 64 kDa
Observed band size: 64 kDa
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