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Cancer Tumor biomarkers Enzymes Phosphatases

Anti-Cdc25B antibody [EPR3459(2)] (ab124819)

Price and availability

298 185 ₸

Availability

Order now and get it on Thursday March 04, 2021

Anti-Cdc25B antibody [EPR3459(2)] (ab124819)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3459(2)] to Cdc25B
  • Suitable for: WB, IP
  • Reacts with: Mouse, Human

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Overview

  • Product name

    Anti-Cdc25B antibody [EPR3459(2)]
    See all Cdc25B primary antibodies
  • Description

    Rabbit monoclonal [EPR3459(2)] to Cdc25B
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    WB
    Mouse
    Human
    See all applications and species data
  • Immunogen

    Synthetic peptide within Human Cdc25B aa 150-250. The exact sequence is proprietary.

  • Positive control

    • K562 cell lysate, U937 cell lysate and THP-1 cell lysate, mouse embryo E17 and NIH3T3 cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.2
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR3459(2)
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Phosphatases
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Phospatases
    • Cancer
    • Cell cycle
    • Kinases/phosphatases
    • Phosphatases

Images

  • Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819)
    Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819)
    All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 0.486 µg/ml

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) cell lysates
    Lane 2 : Mouse embryo E17 lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 0.05 µg/ml

    Predicted band size: 64 kDa
    Observed band size: 64 kDa



    Blocking/diluting Buffer and concentration: 5% NFDM /TBST
  • Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819)
    Western blot - Anti-Cdc25B antibody [EPR3459(2)] (ab124819)
    All lanes : Anti-Cdc25B antibody [EPR3459(2)] (ab124819) at 1/1000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : U937 cell lysate
    Lane 3 : THP-1 cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat-anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 64 kDa
    Observed band size: 64 kDa

  • Anti-Cdc25B antibody [EPR3459(2)] (ab124819)
    Anti-Cdc25B antibody [EPR3459(2)] (ab124819)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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