Anti-BRN3B / POU4F2 antibody (ab128849)
Key features and details
- Rabbit polyclonal to BRN3B / POU4F2
- Suitable for: WB
- Reacts with: Rat
- Isotype: IgG
Overview
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Product name
Anti-BRN3B / POU4F2 antibody
See all BRN3B / POU4F2 primary antibodies -
Description
Rabbit polyclonal to BRN3B / POU4F2 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Rabbit, Horse, Cow, Human, Pig, Chimpanzee, Macaque monkey, Chinese hamster, Orangutan
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Immunogen
Synthetic peptide corresponding to Mouse BRN3B/ POU4F2 aa 200-300 conjugated to keyhole limpet haemocyanin.
Database link: Q63934 -
Positive control
- This antibody gave a positive signal in Rat Retina tissue lysate.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration
Concentration information loading...Purity
Immunogen affinity purifiedClonality
PolyclonalIsotype
IgGResearch areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
Our Abpromise guarantee covers the use of ab128849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application Abreviews Notes WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa). Target
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Function
Transcription factor. May play a role in determining or maintaining the identities of a small subset of visual system neurons. -
Tissue specificity
Brain. Seems to be specific to the retina. -
Sequence similarities
Belongs to the POU transcription factor family. Class-4 subfamily.
Contains 1 homeobox DNA-binding domain.
Contains 1 POU-specific domain. -
Cellular localization
Nucleus speckle. - Information by UniProt
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Database links
- Entrez Gene: 471317 Chimpanzee
- Entrez Gene: 532453 Cow
- Entrez Gene: 5458 Human
- Entrez Gene: 18997 Mouse
- Entrez Gene: 171355 Rat
- Omim: 113725 Human
- SwissProt: Q12837 Human
- SwissProt: Q63934 Mouse
see all -
Alternative names
- Brain specific homeobox/POU domain protein 3B antibody
- Brain-3B antibody
- Brain-specific homeobox/POU domain protein 3B antibody
see all
Images
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Western blot - Anti-BRN3B / POU4F2 antibody (ab128849)Anti-BRN3B / POU4F2 antibody (ab128849) at 1 µg/ml + Rat Retina Tissue Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 23 kDa (possible non-specific binding), 53 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128849 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
References (0)
ab128849 has not yet been referenced specifically in any publications.
Images
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Western blot - Anti-BRN3B / POU4F2 antibody (ab128849)Anti-BRN3B / POU4F2 antibody (ab128849) at 1 µg/ml + Rat Retina Tissue Lysate at 25 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 43 kDa
Observed band size: 43 kDa
Additional bands at: 23 kDa (possible non-specific binding), 53 kDa (possible non-specific binding)
Exposure time: 4 minutesThis blot was produced using a 10% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab128849 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
