Anti-BRN3B / POU4F2 antibody (ab235268)
Key features and details
- Goat polyclonal to BRN3B / POU4F2
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat
- Isotype: IgG
Overview
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Product name
Anti-BRN3B / POU4F2 antibody
See all BRN3B / POU4F2 primary antibodies -
Description
Goat polyclonal to BRN3B / POU4F2 -
Host species
Goat -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Rat pup PND2-6 – minimal cortex brain lysate. IHC-P: Mouse testis tissue. ICC/IF: Post-natal rat pup (PND1) heterogeneous brain cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling BRN3B / POU4F2 with ab235268 at 1/100 dilution (30 minutes at RT), followed by Donkey anti-Goat (HRP) (4µl/ml, 20 minutes at RT). Primary staining is DAB. Counterstained with hematoxylin.
Perform heat mediated antigen retrieval using citrate buffer for 20 minutes. -
Anti-BRN3B / POU4F2 antibody (ab235268) at 1/1000 dilution + Rat pup PND2-6 – minimal cortex brain lysate at 35 µg
Predicted band size: 43 kDa
Exposure time: 12 seconds
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Immunofluorescent analysis of 4% PFA-fixed, 0.3% Triton X-100 permeabilized Post-natal rat pup (PND1) heterogeneous brain cells labeling BRN3B / POU4F2 with ab235268 at 5 µg/ml (Overnight at 4°C), followed by a Donkey anti-Goat IgG DyLight™488 conjugated preadsorbed secondary antibody at 15 µg/ml (1 hour at RT) (green).
Expected localization: Nuclear, nuclear speckle. The nuclear counterstain is DAPI (blue).
Panel A: DAPI.
Panel B: ab235268 + secondary antibody.
Panel C: Merge of panel A and B.
Panel D: Secondary only.