Anti-53BP1 (phospho S25) antibody (ab70323)
Key features and details
- Rabbit polyclonal to 53BP1 (phospho S25)
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-53BP1 (phospho S25) antibody
See all 53BP1 primary antibodies -
Description
Rabbit polyclonal to 53BP1 (phospho S25) -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P MouseHumanWB Human -
Immunogen
A phosphorylated synthetic peptide representing a portion of human 53BP1 around serine 25 (NP_005648.1.)
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-53BP1 (phospho S25) antibody (ab70323)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse teratoma (right) tissues labelling 53BP1 (phospho S24) with ab70323 at 1/1000 (1µg/ml). Detection: DAB.
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All lanes : Anti-53BP1 (phospho S25) antibody (ab70323) at 0.1 µg/ml
Lane 1 : Whole cell lysate from 293T cells, untreated
Lane 2 : Whole cell lysate from 293T cells treated
with NCS (200 ng/ml for 1h)
Lysates/proteins at 50 µg per lane.
Predicted band size: 214 kDa
Observed band size: >220 kDa why is the actual band size different from the predicted?