Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21987] to Pericentrin - BSA and Azide free
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Pericentrin antibody [EPR21987] - BSA and Azide free
    See all Pericentrin primary antibodies

  • Description

    Rabbit monoclonal [EPR21987] to Pericentrin - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    IHC is recommended for human only.

  • Tested applications

    Suitable for: IHC-P, ICC/IF, Flow Cyt, IPmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human breast tissue.

  • General notes

    Ab237034 is the carrier-free version of ab220784. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab237034 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

    Immunofluorescent analysis of 100% Methanol-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in NIH/3T3 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
    Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

    Pericentrin was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 μg (input).
    Lane 2: ab220784 IP in NIH/3T3 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220784 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds

    The 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We use high sensitive ECL substrate.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
    Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

    Pericentrin was immunoprecipitated from 0.35 mg HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HepG2 whole cell lysate 10 μg (input).
    Lane 2: ab220784 IP in HepG2 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220784 in HepG2 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds

    The 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We used a high sensitivity ECL substrate to develop this blot.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Flow Cytometry - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
    Flow Cytometry - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Pericentrin with ab220784 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human thyroid carcinoma (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human pancreas (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

    Immunofluorescent analysis of 100% Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in HeLa cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human mammary epithelial cells (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab220784).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)
    Anti-Pericentrin antibody [EPR21987] - BSA and Azide free (ab237034)

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