Anti-Pericentrin antibody (ab99341)
Key features and details
- Rabbit polyclonal to Pericentrin
- Suitable for: WB, IP, ICC
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Pericentrin antibody
See all Pericentrin primary antibodies -
Description
Rabbit polyclonal to Pericentrin -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, ICCmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide, corresponding to a region within amino acids 175-225 of Human Pericentrin (NP_006022.3).
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Positive control
- HeLa whole cell lysate
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunocytochemistry - Anti-Pericentrin antibody (ab99341)Immunocytochemistry/Immunofluorescence analysis of formaldehyde-fixed asynchronous HeLa cells labelling Pericentrin with ab99341 at 1/1000 (1µg/ml). A DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
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Western blot - Anti-Pericentrin antibody (ab99341)All lanes : Anti-Pericentrin antibody (ab99341) at 0.1 µg/ml
Lane 1 : HeLa cell lysate prepared using NETN lysis buffer
Lane 2 : HEK293T cell lysate prepared using NETN lysis buffer
Lysates/proteins at 50 µg per lane.
Predicted band size: 378 kDaDetection: Chemiluminescence with an exposure time of 30 seconds.
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Immunoprecipitation - Anti-Pericentrin antibody (ab99341)Detection of Pericentrin by Western Blot of Immunoprecipitates in HeLa whole cell lysate (1 mg for IP, 20% of IP loaded) using ab99341 at 3 µg/mg lysate. Anti-Pericentrin antibody ab99342, which recognizes a downstream epitope, was used at 1 µg/ml for subsequent WB detection.
