Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Pericentrin antibody [EPR21987] (ab220784)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21987] to Pericentrin
  • Suitable for: IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Pericentrin antibody [EPR21987]
    See all Pericentrin primary antibodies

  • Description

    Rabbit monoclonal [EPR21987] to Pericentrin

  • Host species

    Rabbit

  • Specificity

    IHC is recommended for human only.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    Human
    IHC-P
    Human
    IP
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • ICC/IF: HeLa and NIH/3T3 cells. IHC-P: Human breast, pancreas and thyroid carcinoma tissues. Flow Cyt: HeLa cells. IP: HepG2 and NIH/3T3 whole cell lysates.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR21987

  • Isotype

    IgG

  • Research areas

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] (ab220784)
    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] (ab220784)

    Immunofluorescent analysis of 100% Methanol-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in HeLa cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] (ab220784)
    Immunocytochemistry/ Immunofluorescence - Anti-Pericentrin antibody [EPR21987] (ab220784)

    Immunofluorescent analysis of 100% Methanol-fixed NIH/3T3 (mouse embyro fibroblast cell line) cells labeling Pericentrin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing centrosome staining in NIH/3T3 cell line.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] (ab220784)
    Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] (ab220784)

    Pericentrin was immunoprecipitated from 0.35 mg HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HepG2 whole cell lysate 10 μg (input).
    Lane 2: ab220784 IP in HepG2 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220784 in HepG2 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds

    The 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We used a high sensitivity ECL substrate to develop this blot.

  • Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] (ab220784)
    Immunoprecipitation - Anti-Pericentrin antibody [EPR21987] (ab220784)

    Pericentrin was immunoprecipitated from 0.35 mg NIH/3T3 (mouse embryonic fibroblast cell line) whole cell lysate with ab220784 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab220784 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection 1/1000 dilution.

    Lane 1: NIH/3T3 whole cell lysate 10 μg (input).
    Lane 2: ab220784 IP in NIH/3T3 whole cell lysate (+).
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab220784 in NIH/3T3 whole cell lysate (-).

    Blocking and dilution buffer and concentration: 5% NFDM/TBST
    Exposure time: 30 seconds

    The 220 kDa band represent an isoform of Pericentrin (PMID: 23060948). We use high sensitive ECL substrate.

  • Flow Cytometry - Anti-Pericentrin antibody [EPR21987] (ab220784)
    Flow Cytometry - Anti-Pericentrin antibody [EPR21987] (ab220784)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling Pericentrin with ab220784 at 1/60 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells incubated with secondary antibody only) (blue). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] (ab220784)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] (ab220784)

    Immunohistochemical analysis of paraffin-embedded human breast tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human mammary epithelial cells (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] (ab220784)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] (ab220784)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human pancreas (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] (ab220784)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Pericentrin antibody [EPR21987] (ab220784)

    Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma tissue labeling Pericentin with ab220784 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Positive staining of centrosomes in human thyroid carcinoma (PMID: 16534625, PMID: 22031837). Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

     

  • Anti-Pericentrin antibody [EPR21987] (ab220784)
    Anti-Pericentrin antibody [EPR21987] (ab220784)

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