Anti-ErbB2 / HER2 (phospho Y1248) antibody (ab131104)
Key features and details
- Rabbit polyclonal to ErbB2 / HER2 (phospho Y1248)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-ErbB2 / HER2 (phospho Y1248) antibody
See all ErbB2 / HER2 primary antibodies -
Description
Rabbit polyclonal to ErbB2 / HER2 (phospho Y1248) -
Host species
Rabbit -
Specificity
ab131104 detects endogenous levels of ErbB2 / HER2 only when phosphorylated at tyrosine 1248.
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Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide corresponding to Human ErbB2/ HER2 conjugated to keyhole limpet haemocyanin. Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of Tyrosine 1248 (
P-E-Y(p)-L-G
) of Human ErbB2/ HER2 (UniProt P04626).
Sequence:P-E-Y(p)-L-G
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Positive control
- MDA cell extract untreated and treated with EGF; MCF7 cells, Human breast carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.88% Sodium chloride
PBS is without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131104 was purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-ErbB2 / HER2 (phospho Y1248) antibody (ab131104)All lanes : Anti-ErbB2 / HER2 (phospho Y1248) antibody (ab131104) at 1/500 dilution
Lane 1 : MDA cell extract untreated
Lane 2 : MDA cell extract treated with EGF
Predicted band size: 137 kDa
Observed band size: 185 kDa why is the actual band size different from the predicted?
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ErbB2 / HER2 (phospho Y1248) antibody (ab131104)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling ErbB2 / HER2 (phospho Y1248) with ab131104 at 1/50 dilution. Right panel was preincubated with blocking peptide.
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Immunocytochemistry/ Immunofluorescence - Anti-ErbB2 / HER2 (phospho Y1248) antibody (ab131104)Immunofluorescence analysis of methanol-fixed MCF7 cells labelling ErbB2 / HER2 (phospho Y1248) with ab131104 at 1/100 dilution.
