SDS3 overexpression 293T lysate (whole cell) (ab94303)
Overview
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Product name
SDS3 overexpression 293T lysate (whole cell) -
General notes
ab94303 is a 293T cell transfected lysate in which Human SDS3 has been transiently over-expressed using a pCMV-SDS3 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more detailed how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Regulatory protein which represses transcription and augments histone deacetylase activity of HDAC1. May have a potential role in tumor suppressor pathways (By similarity). May function in the assembly and/or enzymatic activity of the mSin3A corepressor complex or in mediating interactions between the complex and other regulatory complexes. Tissue specificity: Expressed in various cancer cell ines. Similarity: Belongs to the SDS3 family. Domain: The C-terminus is involved in transcriptional repression by HDAC-independent mechanisms.
Images
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SDS-PAGE - SDS3 overexpression 293T lysate (whole cell) (ab94303)ab94303 at 15μg/lane on an SDS-PAGE gel
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Western blot - SDS3 overexpression 293T lysate (whole cell) (ab94303)All lanes : Anti-SDS3 antibody (ab89345) at 1/500 dilution
Lane 1 :SDS3 overexpression 293T lysate (whole cell) (ab94303)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution