Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3986] to Menin - BSA and Azide free
  • Suitable for: IP, ICC/IF, IHC-P, WB
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-Menin antibody [EPR3986] - BSA and Azide free
    See all Menin primary antibodies

  • Description

    Rabbit monoclonal [EPR3986] to Menin - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, ICC/IF, IHC-P, WBmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IP: Jurkat whole cell lysate.

  • General notes

    ab239910 is the carrier-free version of ab92443 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab239910 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)

    ab92443, at 1/100 dilution, staining Menin in paraffin-embedded (A) Human colon tissue and (B) Human papillary carcinoma of thyroid gland by Immunohistochemistry.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92443).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)
    Immunocytochemistry/ Immunofluorescence - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)
    ab92443, at 1/250 dilution, staining Menin in HeLa cells by Immunofluorescence.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92443).

  • Immunoprecipitation - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)
    Immunoprecipitation - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)

    This data was developed using ab92443, the same antibody clone in a different buffer formulation.
    Purified ab92443 at 1/50 dilution (2µg) immunoprecipitating Menin in Jurkat whole cell lysate.
    Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
    Lane 2 (+): ab92443 + Jurkat whole cell lysate.
    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab92443 in Jurkat whole cell lysate.
    VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
    Blocking Buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM/TBST.
    Observed band size: 75 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)

    ab92443 showing positive staining in Breast carcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92443).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)

    ab92443 showing positive staining in Colonic adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92443).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)

    ab92443 showing positive staining in Lung adenocarcinoma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92443).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)

    ab92443 showing positive staining in Glioma tissue.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab92443).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)
    Anti-Menin antibody [EPR3986] - BSA and Azide free (ab239910)

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