Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - Low endotoxin, Azide free
  • Suitable for: WB, IHC-P
  • Reacts with: Human

Overview

  • Product name

    Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free
    See all Somatostatin Receptor 2 primary antibodies

  • Description

    Rabbit monoclonal [UMB1] to Somatostatin Receptor 2 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-Fr

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat

  • Immunogen

    This information is proprietary to Abcam and/or its suppliers.
    (Peptide available as ab171899)

  • Positive control

    • WB: U937, INS-1, and sstr2 transfected HEK293 cell lysates. IHC-P: Human pancreas & brain tissue

  • General notes

    ab216649 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649)
    Immunocytochemistry/ Immunofluorescence - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649) Lehmann A. et al. PLoS One. 2014 Mar 17;9(3):e91526. doi: 10.1371/journal.pone.0091526. eCollection 2014.

    Agonist-induced internalization of sst2 (Somatostatin Receptor 2)  and sst5 (Somatostatin Receptor 5) tail-swap mutants

    Stably transfected HEK-293 cells were treated with 1 µM SS-14 for 0, 15 or 30 min. Cells were then fixed, stained with the anti-sst2 {UMB-1} (ab134152) or anti-sst5 antibody {UMB-4} (ab109495) and examined by confocal microscopy. Shown are representative images from one of at least three independent experiments. Scale bar, 20 µm.

    Cells were cells were fixed with 4% paraformaldehyde and 0.2% picric acid in phosphate buffer (pH 6.9) for 30 min at room temperature and washed several times. Cells were them permeabilized prior to incubation with the respective primary antibodies. Alexa® 488-conjugated secondary antibodies were used.

    (After Figure 2A of Lehmann et al)

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649)

    Immunohistochemical analysis of paraffin embedded human pancreas tissue labeled with ab134152 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649)

    Immunohistochemical analysis of paraffin embedded human brain tissue labeled with ab134152 at 1/100 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab134152).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649)
    Anti-Somatostatin Receptor 2 antibody [UMB1] - Low endotoxin, Azide free (ab216649)

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