Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free (ab227107)
![Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free (ab227107)](https://www.abcam.com/ps/products/227/ab227107/Images/ab227107-2-ab207612261162207612IP.jpg "Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free (ab227107)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR19662] to Beclin 1 - BSA and Azide free
- Suitable for: WB, IP
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free
See all Beclin 1 primary antibodies -
Description
Rabbit monoclonal [EPR19662] to Beclin 1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human fetal brain, fetal heart and fetal kidney lysates; HeLa, HepG2, 293, MCF7, Jurkat, Raji, C6, RAW264.7, PC-12, NIH/3T3, A20 and C2C12 whole cell lysates; rat brain, kidney and spleen lysates; mouse brain, heart and spleen lysates. IP: HeLa whole cell lysate.
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General notes
Ab227107 is the carrier-free version of ab207612. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab227107 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR19662 -
Isotype
IgG -
Research areas
Images
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Immunoprecipitation - Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free (ab227107)
Beclin 1 was immunoprecipitated from 0.35 mg of HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate with ab207612 at 1/30 dilution.
Western blot was performed from the immunoprecipitate using ab207612 at 1/1000 dilution.
VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.
Lane 1: HeLa whole cell lysate 10µg (Input).
Lane 2: ab207612 IP in HeLa whole cell lysate.
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab207612 in HeLa whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab207612).
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![Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free (ab227107)](https://www.abcam.com/ps/products/227/ab227107/Images/ab227107-5-benefits-of-recombinant-antibodies.png)
Anti-Beclin 1 antibody [EPR19662] - BSA and Azide free (ab227107)
