Anti-Beclin 1 antibody (ab62557)
Key features and details
- Rabbit polyclonal to Beclin 1
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-Beclin 1 antibody
See all Beclin 1 primary antibodies -
Description
Rabbit polyclonal to Beclin 1 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF HumanIF MouseIHC-P MouseRatHumanWB MouseRatHuman
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Immunogen
Synthetic peptide corresponding to Human Beclin 1 (N terminal). ab62557 was raised against a 17 amino acid synthetic peptide from near the amino terminus of human Beclin-1. The immunogen is located within amino acids 40 - 90 of Beclin-1.
Database link: Q14457 -
Positive control
- WB: HEK-293, A549, HeLa, HepG2, K562, MCF7 and SK-N-SH cell lines. Mouse heart, brain and colon tissue. Rat liver, lung, brain and colon tissue. IHC-P: Human brain tissue, mouse kidney and rat liver and spleen tissues. IF: Mouse brain. ICC/IF: HeLa cells.
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General notes
For Western blot protocol see the applications section.
For information on sample preparation for western blot, please refer here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Beclin 1 antibody (ab62557)All lanes : Anti-Beclin 1 antibody (ab62557) at 1 µg/ml
Lane 1 : 293 human cell line
Lane 2 : A549 human cell line
Lane 3 : HeLa cell line
Lane 4 : HepG2 cell line
Lane 5 : K562 cell line
Lane 6 : MCF7 cell line
Lane 7 : SK-N-SH cell line
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/1000 dilution
Predicted band size: 52 kDaGel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue labelling Beclin 1 with ab6257 at 1 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0, microwave, for 20min. Streptavidin-HRP Kit (Rabbit IgG) was used as the secondary antibody.
blocking condition: 5% BSA at Room Temperature for 1 hour
Chromagen system: DAB
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Immunocytochemistry/ Immunofluorescence - Anti-Beclin 1 antibody (ab62557)Immunofluorescence analysis of HeLa cells labelling Beclin 1 with ab62557 at 1/100 dilution. Cells were fixed with 4% paraformaldehyde at room temperature for 15 minutes and was permeabilized with 0.1% Triton X-100 at room temperature for 4 minutes. Rabbit IgG antibody (Alexa Fluor 488) at 1/2000 was used as the secondary antibody (green). Nuclear DNA was labelled with DAPI (blue).
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat liver tissue labelling Beclin 1 with ab6257 at 1 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0, microwave, for 20min. Streptavidin-HRP Kit (Rabbit IgG) was used as the secondary antibody.
blocking condition: 5% BSA at Room Temperature for 1 hour
Chromagen system: DAB
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Western blot - Anti-Beclin 1 antibody (ab62557)All lanes : Anti-Beclin 1 antibody (ab62557) at 1 µg/ml
Lane 1 : Mouse heart tissue
Lane 2 : Mouse brain tissue
Lane 3 : Mouse colon tissue
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 52 kDaGel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
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Western blot - Anti-Beclin 1 antibody (ab62557)All lanes : Anti-Beclin 1 antibody (ab62557) at 1 µg
Lane 1 : Rat liver tissue
Lane 2 : Rat heart tissue
Lane 3 : Rat lung tissue
Lane 4 : Rat brain tissue
Lane 5 : Rat ovary tissue
Lane 6 : Rat colon tissue
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Rabbit IgG antibody (HRP) at 1/10000 dilution
Predicted band size: 52 kDaGel: 10%
Running condition: 130V, 1.5hrs
Transfer condition: Wet, 250mA, 2 hrs (NC membrane)
Blocking condition: 5% non-fat milk in TBS at 4 overnight
Primary antibody incubation: RT, 1hr
Secondary antibody incubation: RT, 1hr
Washing condition: 5 ml TBST, 4 x 5min
Exposure system: GE ECL 10 sec
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling Beclin 1 with ab6257 at 1 µg/mL. Heat mediated antigen retrieval was performed using citrate buffer, pH 6.0, microwave, for 20min. Streptavidin-HRP Kit (Rabbit IgG) was used as the secondary antibody.
blocking condition: 5% BSA at Room Temperature for 1 hour
Chromagen system: DAB
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Immunofluorescence - Anti-Beclin 1 antibody (ab62557)ab62557 at 20 µg/mL staining Beclin 1 (green) in mouse brain tissue by Immunofluorescence. DAPI staining in blue.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody (ab62557)Paraffin-embedded human brain tissue stained for Beclin 1 using ab62557 at 2.5 µg/ml in immunohistochemical analysis.
