ELMO2 overexpression 293T lysate (whole cell) (ab94207)
Overview
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Product name
ELMO2 overexpression 293T lysate (whole cell) -
General notes
ab94207 is a 293T cell transfected lysate in which Human ELMO2 has been transiently over-expressed using a pCMV-ELMO2 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
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Tested applications
Suitable for: WBmore details
Properties
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Mycoplasma free
Yes -
Form
Liquid -
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Research areas
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Background
Function: Involved in cytoskeletal rearrangements required for phagocytosis of apoptotic cells and cell motility. Acts in assocation with DOCK1 and CRK. Was initially proposed to be required in complex with DOCK1 to activate Rac Rho small GTPases. May enhance the guanine nucleotide exchange factor (GEF) activity of DOCK1. Tissue specificity: Widely expressed, with a higher expression in skeletal muscle, kidney and placenta. Similarity: Contains 1 ELMO domain. Contains 1 PH domain.
Images
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SDS-PAGE - ELMO2 overexpression 293T lysate (whole cell) (ab94207)ab94207 at 15µg/lane on an SDS-PAGE gel.
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Western blot - ELMO2 overexpression 293T lysate (whole cell) (ab94207)All lanes : Anti-ELMO2 antibody (ab57557) at 1/500 dilution
Lane 1 :ELMO2 overexpression 293T lysate (whole cell) (ab94207)
Lane 2 : 293T non-transfected lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
