Anti-Beclin 1 antibody [OTI4A10] (ab118148)
![Anti-Beclin 1 antibody [OTI4A10] (ab118148)](https://www.abcam.com/ps/products/118/ab118148/Images/ab118148-391735-anti-beclin-1-antibody-oti4a10-western-blot-hela-wt-hela-becn1-knockout.jpg "Anti-Beclin 1 antibody [OTI4A10] (ab118148)")
Key features and details
- Mouse monoclonal [OTI4A10] to Beclin 1
- Suitable for: WB
- Knockout validated
- Reacts with: Human
- Isotype: IgG2a
Overview
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Product name
Anti-Beclin 1 antibody [OTI4A10]
See all Beclin 1 primary antibodies -
Description
Mouse monoclonal [OTI4A10] to Beclin 1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Recombinant full length protein corresponding to Human Beclin 1. Produced in HEK-293T cells. NP_003757
Database link: Q14457 -
Positive control
- WB: Beclin 1 transfected HEK-293T cell lysate; HeLa cell lysate.
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General notes
The clone number has been updated from 4A10 to OTI4A10, both clone numbers name the same clone.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading... -
Purity
Affinity purified -
Purification notes
Purified from cell culture supernatant by affinity chromatography -
Clonality
Monoclonal -
Clone number
OTI4A10 -
Isotype
IgG2a -
Research areas
Images
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Western blot - Anti-Beclin 1 antibody [OTI4A10] (ab118148)All lanes : Anti-Beclin 1 antibody [OTI4A10] (ab118148) at 1/1000 dilution
Lane 1 : Wild-type HeLa cell lysate
Lane 2 : BECN1 knockout HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Performed under reducing conditions.
Predicted band size: 52 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?Lanes 1 - 2: Merged signal (red and green). Green - ab118148 observed at 60 kDa. Red - loading control, ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) observed at 37kDa.
ab118148 was shown to react with Beclin 1 in wild-type HeLa cells in western blot. Loss of signal was observed when BECN1 knockout sample was used. Wild-type HeLa and BECN1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in non-mammalian (TBS-based) blocking solution before incubation with ab118148 and ab181602 (Rabbit Anti-GAPDH antibody [EPR16891]) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-Beclin 1 antibody [OTI4A10] (ab118148)](https://www.abcam.com/ps/products/118/ab118148/Images/ab118148-130276-anti-beclin-1-antibody-oti4a10-western-blot.jpg)
Western blot - Anti-Beclin 1 antibody [OTI4A10] (ab118148)All lanes : Anti-Beclin 1 antibody [OTI4A10] (ab118148) at 1/2000 dilution
Lane 1 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control
Lane 2 : HEK-293T cells transfected with pCMV6-ENTRY Beclin 1
Lysates/proteins at 5 µg per lane.
Predicted band size: 52 kDa
