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Cancer Oncoproteins/suppressors Tumor suppressors

Human Beclin-1 Antibody Pair - BSA and Azide free (ab256743)

Price and availability

670 ₸

Availability

Order now and get it on Tuesday March 09, 2021

Human Beclin-1 Antibody Pair - BSA and Azide free (ab256743)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Unconjugated capture and detector antibodies
  • Adaptable to any antibody pair-based assay format
  • Antibody concentration ~ 1 mg/ml
  • BSA and azide free buffer - ready for conjugation
  • Reacts with: Human

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Overview

  • Product name

    Human Beclin-1 Antibody Pair - BSA and Azide free
    See all Beclin 1 kits
  • Assay type

    ELISA set
  • Range

    1.562 ng/ml - 100 ng/ml
  • Species reactivity

    Reacts with: Human
  • Product overview

    The Antibody Pair can be used to quantify Human Beclin-1. BSA and Azide free antibody pairs include unconjugated capture and detector antibodies suitable for sandwich ELISAs. The antibodies are provided at an approximate concentration of 1 mg/ml as measured by the protein A280 method. The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Both capture and detector antibodies are rabbit monoclonal antibodies delivering consistent, specific, and sensitive results.


    For additional information on the performance of the antibody pair, see the equivalent SimpleStep ELISA® Kit (ab254511), which uses the same antibodies. However, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits. Please note that the range provided for the pairs is only an estimation based on the performance of the related product using the same antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody pair in your assay.


    Download SDS here.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Reagents

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Carrier free

    Yes
  • Components 10 x 96 tests
    Human Beclin-1 Capture Antibody (unconjugated) 1 x 100µg
    Human Beclin-1 Detector Antibody (unconjugated) 1 x 100µg
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Microbiology
    • Interspecies Interaction
    • Host Immune Response
    • Cancer
    • Signal transduction
    • Autophagy
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Autophagy and mitophagy
    • Cancer
    • Cell Death
    • Autophagy
    • Signal Transduction
  • Function

    Plays a central role in autophagy (By similarity). May play a role in antiviral host defense. Protects against infection by a neurovirulent strain of Sindbis virus.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the beclin family.
  • Cellular localization

    Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.
  • Target information above from: UniProt accession Q14457 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • APG6
    • ATG 6
    • ATG6
    • ATG6 autophagy related 6 homolog
    • Bcl-2-interacting protein beclin
    • Beclin 1 (coiled coil moesin like BCL2 interacting protein)
    • Beclin 1 autophagy related
    • Beclin-1
    • Beclin1
    • BECN 1
    • Becn1
    • BECN1_HUMAN
    • Coiled coil myosin like BCL2 interacting protein
    • Coiled-coil myosin-like BCL2-interacting protein
    • GT197
    • Protein GT197
    • VPS 30
    • VPS30
    see all
  • Database links

    • Entrez Gene: 8678 Human
    • Omim: 604378 Human
    • SwissProt: Q14457 Human
    • Unigene: 716464 Human

    Images

    • Sandwich ELISA - Human Beclin-1 Antibody Pair - BSA and Azide free (ab256743)
      Sandwich ELISA - Human Beclin-1 Antibody Pair - BSA and Azide free (ab256743)
      To learn more about the advantages of recombinant antibodies see here.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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