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Human Beclin-1 ELISA Kit (ab254511)

Key features and details

  • One-wash 90 minute protocol
  • Sensitivity: 671 ng/ml
  • Range: 1.562 ng/ml - 100 ng/ml
  • Sample type: Cell culture extracts
  • Detection method: Colorimetric
  • Assay type: Sandwich (quantitative)
  • Reacts with: Human

Overview

  • Product name

    Human Beclin-1 ELISA Kit
    See all Beclin 1 kits

  • Detection method

    Colorimetric

  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Extract 24 5.2%
    Inter-assay
    Sample n Mean SD CV%
    Extract 3 1.4%

  • Sample type

    Cell culture extracts

  • Assay type

    Sandwich (quantitative)

  • Sensitivity

    671 ng/ml

  • Range

    1.562 ng/ml - 100 ng/ml

  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 109 99% - 117%

  • Assay time

    1h 30m

  • Assay duration

    One step assay

  • Species reactivity

    Reacts with: Human

  • Product overview

    Human Beclin-1 ELISA Kit (ab254511) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of Beclin-1 protein in cell culture extracts. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human Beclin-1 with 671 ng/ml sensitivity.


    SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:


            - Single-wash protocol reduces assay time to 90 minutes or less
            - High sensitivity, specificity and reproducibility from superior antibodies
            - Fully validated in biological samples
            - 96-wells plate breakable into 12 x 8 wells strips


    A 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.

  • Notes

    Beclin-1, also known as BECN1, plays a key role in the regulation of autophagy and cell death. Together with PI3KC3, Beclin-1 nucleates autophagosome formation and is essential for proper degradation of cellular components. Improper beclin-1 signaling is associated with schizophrenia, tumorigenesis, and neurodegeneration.

    Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
    It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

  • Platform

    Pre-coated microplate (12 x 8 well strips)

Properties

Images

  • SimpleStep ELISA Protocol Diagram
    SimpleStep ELISA Protocol Diagram

    SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.

     

  • Example of human Beclin-1 standard curve in 1X Cell Extraction Buffer PTR.
    Example of human Beclin-1 standard curve in 1X Cell Extraction Buffer PTR.

    The Beclin-1 standard curve was prepared as described in Section 10. Raw data values are shown in the table. Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated concentrations of native Beclin-1 in human MCF-7 cell extract based on a 1,000 µg/mL extract load.
    Interpolated concentrations of native Beclin-1 in human MCF-7 cell extract based on a 1,000 µg/mL extract load.

    The concentrations of Beclin-1 were measured in duplicate and interpolated from the Beclin-1 standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean Beclin-1 concentration was determined to be 10.03 ng/mL.

  • Sandwich ELISA - Human Beclin-1 ELISA Kit (ab254511)
    Sandwich ELISA - Human Beclin-1 ELISA Kit (ab254511)
    To learn more about the advantages of recombinant antibodies see here.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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