Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR20473] to Beclin 1 - BSA and Azide free
  • Suitable for: WB, IHC-P, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free
    See all Beclin 1 primary antibodies

  • Description

    Rabbit monoclonal [EPR20473] to Beclin 1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IHC-P, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human kidney tissue.

  • General notes

    Ab232461 is the carrier-free version of ab210498. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232461 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue labeling Beclin 1 with ab210498 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Granularly cytoplasmic staining on tumor cells of human breast cancer is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    We don’t recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210498).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)
    Immunoprecipitation - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)

    Beclin 1 was immunoprecipitated from 0.35 mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab210498 at 1/30 dilution.

    Western blot was performed from the immunoprecipitate using ab210498 at 1/1000 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: HepG2 whole cell lysate, 10 µg (Input).

    Lane 2: ab210498 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab210498 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210498).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)

    Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling Beclin 1 with ab210498 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Granularly cytoplasmic staining on human kidney is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    We don’t recommend this antibody for mouse and rat in IHC. In our hands most mouse and rat tissues showed nuclear staining.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab210498).

    Heat mediated antigen retrieval was performed with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)
    Anti-Beclin 1 antibody [EPR20473] - BSA and Azide free (ab232461)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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