Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR427(N)] to mTOR (phospho S2481) - BSA and Azide free
  • Suitable for: Dot blot, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free
    See all mTOR primary antibodies

  • Description

    Rabbit monoclonal [EPR427(N)] to mTOR (phospho S2481) - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HeLa (+/- grown in serum-free media overnight, then treated with 200nM PMA for 4 hours) cells.

  • General notes

    Ab232486 is the carrier-free version of ab137133. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab232486 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)
    Dot Blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)

    Dot blot analysis of mTOR (phospho S2481) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2481) phospho peptide with purified ab137133 at a dilution of 1/1000 (1.21ug/ml). A Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.

    Blocking buffer: 5% NFDM/TBST
    Diluting buffer: 5% NFDM /TBST

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137133).

  • Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)
    Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)
    All lanes : Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133) at 1/1000 dilution

    Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate
    Lane 2 : HeLa grown in serum-free media overnight, then treated with 200 nM PMA for 4 hours, whole cell lysate
    Lane 3 : HeLa grown in serum-free media overnight, then treated with 200 nM PMA for 4 hours, whole cell lysate. Then the membrane was incubated with alkaline phosphatase.

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 289 kDa
    Observed band size: 289 kDa



    Blocking Buffer: 5% NFDM/TBST.
    Diluting Buffer: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137133).

  • Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)
    Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Alternative products to Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)

Clear all

Recently viewed products

© 2021 Astana Biomed Group LLP. All rights reserved.