Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)
![Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)](https://www.abcam.com/ps/products/232/ab232486/Images/ab232486-326720-anti-mtor-phospho-s2481-antibody-epr427n-dot-blot.jpg "Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR427(N)] to mTOR (phospho S2481) - BSA and Azide free
- Suitable for: Dot blot, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free
See all mTOR primary antibodies -
Description
Rabbit monoclonal [EPR427(N)] to mTOR (phospho S2481) - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HeLa (+/- grown in serum-free media overnight, then treated with 200nM PMA for 4 hours) cells.
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General notes
Ab232486 is the carrier-free version of ab137133. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab232486 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR427(N) -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)
Dot blot analysis of mTOR (phospho S2481) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2481) phospho peptide with purified ab137133 at a dilution of 1/1000 (1.21ug/ml). A Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.
Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM /TBSTThis data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137133).
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![Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)](https://www.abcam.com/ps/products/232/ab232486/Images/ab232486-308960-anti-mtor-phospho-s2481-antibody-epr427n-western-blot.jpg)
Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)All lanes : Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133) at 1/1000 dilution
Lane 1 : HeLa (human cervix adenocarcinoma epithelial cell line) whole cell lysate
Lane 2 : HeLa grown in serum-free media overnight, then treated with 200 nM PMA for 4 hours, whole cell lysate
Lane 3 : HeLa grown in serum-free media overnight, then treated with 200 nM PMA for 4 hours, whole cell lysate. Then the membrane was incubated with alkaline phosphatase.
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 289 kDa
Observed band size: 289 kDaBlocking Buffer: 5% NFDM/TBST.
Diluting Buffer: 5% NFDM/TBST.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137133).
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![Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)](https://www.abcam.com/ps/products/232/ab232486/Images/ab232486-3-benefits-of-recombinant-antibodies.png)
Anti-mTOR (phospho S2481) antibody [EPR427(N)] - BSA and Azide free (ab232486)
