Anti-mTOR (phospho T2446) antibody (ab63552)
Key features and details
- Rabbit polyclonal to mTOR (phospho T2446)
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-mTOR (phospho T2446) antibody
See all mTOR primary antibodies -
Description
Rabbit polyclonal to mTOR (phospho T2446) -
Host species
Rabbit -
Specificity
Detects endogenous levels of mTOR only when phosphorylated at threonine 2446. -
Tested Applications & Species
See all applications and species dataApplication Species WB Mouse
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Immunogen
Synthetic phosphopeptide derived from human mTOR around the phosphorylation site of threonine 2446 (T-R-TP-D-S).
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-mTOR (phospho T2446) antibody (ab63552)All lanes : Anti-mTOR (phospho T2446) antibody (ab63552) at 1/500 dilution
Lane 1 : extracts from NIH/3T3 cells,
treated with Insulin (0.01U/ml, 15mins)
Lane 2 : extracts from NIH/3T3 cells,
treated with Insulin (0.01U/ml, 15mins) with immunizing peptide at 10 µg
Lysates/proteins at 30 µg per lane.
Predicted band size: 289 kDa
Observed band size: 289 kDa
