Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸

Order now and get it on Tuesday March 09, 2021

Anti-mTOR antibody [EPR390(N)] (ab134903)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR390(N)] to mTOR
Suitable for: WB
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-mTOR antibody [EPR390(N)]
See all mTOR primary antibodies
Description

Rabbit monoclonal [EPR390(N)] to mTOR
Host species

Rabbit
Specificity

Expression levels of the target protein vary with sample type and some optimisation may be required.
Tested Applications & Species

Application Species
WB
Mouse

Rat

Human

See all applications and species data
Immunogen

Synthetic peptide corresponding to residues near the N terminus of Human mTOR (UniProt ID: P42345).
Positive control
- WB: Human brain, heart, liver and kidney lysates, Mouse brain heart, liver and kidney lysates, Rat brain, heart and kidney lysates, HeLa, Jurkat, MCF7, K562, Raji, HepG2, and HEK-293T cell lysates
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Mouse Rat Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR390(N)
Isotype

IgG
Research areas

Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)

All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/10000 dilution

Lane 1 : Wild-type HEK293T whole cell lysate
Lane 2 : MTOR knockout HEK293T whole cell lysate
Lane 3 : K562 whole cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 289 kDa

Lanes 1 - 3: Merged signal (red and green). Green - ab134903 observed at 289 kDa. Red - loading control, ab130007, observed at 130 kDa.

ab134903 was shown to specifically react with mTOR in wild-type HEK293T cells as signal was lost in MTOR knockout cells. Wild-type and MTOR knockout samples were subjected to SDS-PAGE. Ab134903 and ab130007 (Mouse anti-Vinculin loading control) were incubated overnight at 4°C at 1/10000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)

All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : MYC knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 289 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab32072 observed at 57 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab32072 was shown to react with MYC in wild-type HEK-293T. Loss of signal was observed when knockout sample ab263850 was used. Wild-type and MYC knockout samples were subjected to SDS-PAGE. ab32072 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4^°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)

All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/10000 dilution

Lane 1 : Wild-type HEK-293 cell lysate
Lane 2 : MTOR knockout HEK-293 cell lysate
Lane 3 : Wild-type HEK-293T cell lysate
Lane 4 : MTOR knockout HEK-293T cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 289 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab134903 observed at 289 kDa. Red - loading control, ab130007 observed at 125 kDa.

ab134903 was shown to react with mTOR in wild-type HEK-293T cells. Loss of signal was observed when knockout cell line ab255411 (knockout cell lysate ab263789) was used. Wild-type and mTOR knockout samples were subjected to SDS-PAGE. ab134903 and Anti-Vinculin antibody [VIN-54] (ab130007) were incubated overnight at 4°C at 1 in 10000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)

All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/1000 dilution

Lane 1 : MCF7 (Human breast adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : HepG2 (Human hepatocellular carcinoma epithelial cell) whole cell lysates
Lane 3 : Human brain lysates
Lane 4 : Mouse brain lysates
Lane 5 : Rat brain lysates
Lane 6 : Human liver lysates
Lane 7 : Mouse liver lysates
Lane 8 : Human heart lysates
Lane 9 : Mouse heart lysates
Lane 10 : Rat heart lysates
Lane 11 : Human kidney lysates
Lane 12 : Mouse kidney lysates
Lane 13 : Rat kidney lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 289 kDa
Observed band size: 290 kDa
why is the actual band size different from the predicted?

Blocking buffer and concentration 5% NFDM/TBST
Diluting buffer and concentration 5% NFDM/TBST

Exposure time:
Lane 1 to 5: 80 seconds
Lane 6 to 13: 180 seconds

This antibody detects non-specific bands. It doesn’t detect the target band in some mouse and rat tissues.
Western blot - Anti-mTOR antibody [EPR390(N)] (ab134903)

All lanes : Anti-mTOR antibody [EPR390(N)] (ab134903) at 1/10000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : K562 cell lysate
Lane 3 : Raji cell lysate
Lane 4 : HepG2 cell lysate
Lane 5 : 293T cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution

Predicted band size: 289 kDa
Observed band size: 289 kDa
Anti-mTOR antibody [EPR390(N)] (ab134903)

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