Anti-mTOR (phospho S2448) antibody (ab131538)
Key features and details
- Rabbit polyclonal to mTOR (phospho S2448)
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-mTOR (phospho S2448) antibody
See all mTOR primary antibodies -
Description
Rabbit polyclonal to mTOR (phospho S2448) -
Host species
Rabbit -
Specificity
ab131538 detects endogenous levels of mTOR only when phosphorylated at serine 2448. -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic phosphopeptide conjugated to KLH, derived from around the phosphorylation site of serine 2448 (
T-D-S(p)-Y-S
) of Human mTOR (NP_004949.1). -
Positive control
- MCF cells; 293 cell extract untreated or PMA-treated; Human breast carcinoma tissue
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.88% Sodium chloride, 49% PBS, 50% Glycerol (glycerin, glycerine)
PBS without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab131538 was purified by affinity chromatography using epitope-specific phosphopeptide. Non-phosphospecific antibodies were removed by chromatography using non-phosphopeptide. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-mTOR (phospho S2448) antibody (ab131538)All lanes : Anti-mTOR (phospho S2448) antibody (ab131538) at 1/500 dilution
Lane 1 : 293 cell extract (untreated)
Lane 2 : 293 cell extract (PMA-treated)
Predicted band size: 289 kDa
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Immunocytochemistry/ Immunofluorescence - Anti-mTOR (phospho S2448) antibody (ab131538)Immunofluorescence analysis of methanol-fixed MCF cells labelling mTOR (phospho S2448) with ab131538 at 1/100 dilution. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-mTOR (phospho S2448) antibody (ab131538)Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labelling mTOR (phospho S2448) with ab131538 at 1/50 dilution. Right panel was preincubated with blocking peptide.
