Antibodies, Proteins, Kits and Reagents for Life Science

291 484 ₸ Product size

100 µl 291 484 ₸

Order now and get it on Wednesday March 10, 2021

Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR427(N)] to mTOR (phospho S2481)
Suitable for: WB, Dot blot
Reacts with: Human

Product name

Anti-mTOR (phospho S2481) antibody [EPR427(N)]
See all mTOR primary antibodies
Description

Rabbit monoclonal [EPR427(N)] to mTOR (phospho S2481)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

corresponding to Human mTOR.
(Peptide available as ab187360, ab187361)
Positive control
- WB: HEK-293 and HeLa cell lysates.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR427(N)
Isotype

IgG
Research areas

Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)

All lanes : Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133) at 1/1000 dilution (Purified)

Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate
Lane 3 : HeLa grown in serum-free media overnight, then treated with 200nM PMA for 4 hours whole cell lysate. Then the membrane was incubated with alkaline phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 289 kDa
Observed band size: 289 kDa

Blocking Buffer: 5% NFDM/TBST
Diluting Buffer: 5% NFDM/TBST
Dot Blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)

Dot blot analysis of mTOR (phospho S2481) phospho peptide (Lane 1) and mTOR non-phospho peptide (Lane 2) labeling mTOR (phospho S2481) phospho peptide with purified ab137133 at a dilution of 1/1000 (1.21ug/ml). A Goat Anti-Rabbit IgG (H+L) Peroxidase conjugated (ab97051) was used as the secondary antibody at a dilution of 1/20,000 dilution.

Blocking buffer: 5% NFDM/TBST
Diluting buffer: 5% NFDM /TBST
Western blot - Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)

All lanes : Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133) at 1/1000 dilution

Lane 1 : HEK-293 (human epithelial cell line from embryonic kidney) cell lysate, untreated
Lane 2 : HEK-293 cell lysate, treated with Lambda Phosphatase

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size: 289 kDa
Anti-mTOR (phospho S2481) antibody [EPR427(N)] (ab137133)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com