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Anti-mTOR (phospho S2481) antibody (ab45996)

Key features and details

  • Rabbit polyclonal to mTOR (phospho S2481)
  • Suitable for: ICC/IF, ELISA
  • Reacts with: Human
  • Isotype: IgG

Overview

  • Product name

    Anti-mTOR (phospho S2481) antibody
    See all mTOR primary antibodies

  • Description

    Rabbit polyclonal to mTOR (phospho S2481)

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC/IF, ELISAmore details

  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Zebrafish

  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 2450 to the C-terminus of Human mTOR, phosphorylated at S2481.

    Read Abcam's proprietary immunogen policy (Peptide available as ab46195.)

Properties

Applications

Our Abpromise guarantee covers the use of ab45996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000. This antibody gave a positive result in ELISA against the immunizing peptide (ab46195). It gave a negative result in ELISA against the non-modified equivalent peptide (ab61979). This indicates that it is specific for the modified peptide. See figure below.
ELISA Use at an assay dependent concentration.

Target

  • Function

    Kinase subunit of both mTORC1 and mTORC2, which regulates cell growth and survival in response to nutrient and hormonal signals. mTORC1 is activated in response to growth factors or amino-acids. Growth factor-stimulated mTORC1 activation involves AKT1-mediated phosphorylation of TSC1-TSC2, which leads to the activation of the RHEB GTPase that potently activates the protein kinase activity of mTORC1. Amino-acid-signaling to mTORC1 requires its relocalization to the lysosomes mediated by the Ragulator complex and the Rag GTPases. Activated mTORC1 up-regulates protein synthesis by phosphorylating key regulators of mRNA translation and ribosome synthesis. mTORC1 phosphorylates EIF4EBP1 and releases it from inhibiting the elongation initiation factor 4E (eiF4E). mTORC1 phosphorylates and activates S6K1 at 'Thr-421', which then promotes protein synthesis by phosphorylating PDCD4 and targeting it for degradation. Phosphorylates MAF1 leading to attenuation of its RNA polymerase III-repressive function. mTORC2 is also activated by growth. factors, but seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'.

  • Tissue specificity

    Expressed in numerous tissues, with highest levels in testis.

  • Sequence similarities

    Belongs to the PI3/PI4-kinase family.
    Contains 1 FAT domain.
    Contains 1 FATC domain.
    Contains 7 HEAT repeats.
    Contains 1 PI3K/PI4K domain.

  • Post-translational
    modifications

    Autophosphorylated; when part of mTORC1 or mTORC2.

  • Cellular localization

    Endoplasmic reticulum membrane. Golgi apparatus membrane. Mitochondrion outer membrane. Lysosome. Cytoplasm. Nucleus > PML body. Shuttles between cytoplasm and nucleus. Accumulates in the nucleus in response to hypoxia (By similarity). Targeting to lysosomes depends on amino acid availability and RRAGA and RRAGB.
  • Information by UniProt

  • Database links

    see all

  • Alternative names

    • dJ576K7.1 (FK506 binding protein 12 rapamycin associated protein 1) antibody
    • FK506 binding protein 12 rapamycin associated protein 1 antibody
    • FK506 binding protein 12 rapamycin associated protein 2 antibody
    • FK506 binding protein 12 rapamycin complex associated protein 1 antibody
    • FK506-binding protein 12-rapamycin complex-associated protein 1 antibody
    • FKBP rapamycin associated protein antibody
    • FKBP12 rapamycin complex associated protein antibody
    • FKBP12-rapamycin complex-associated protein 1 antibody
    • FKBP12-rapamycin complex-associated protein antibody
    • FLJ44809 antibody
    • FRAP antibody
    • FRAP1 antibody
    • FRAP2 antibody
    • Mammalian target of rapamycin antibody
    • Mechanistic target of rapamycin antibody
    • mTOR antibody
    • MTOR_HUMAN antibody
    • OTTHUMP00000001983 antibody
    • RAFT1 antibody
    • Rapamycin and FKBP12 target 1 antibody
    • Rapamycin associated protein FRAP2 antibody
    • Rapamycin target protein 1 antibody
    • Rapamycin target protein antibody
    • RAPT1 antibody
    • Serine/threonine-protein kinase mTOR antibody
    see all

Images

  • ELISA - Anti-mTOR (phospho S2481) antibody (ab45996)
    ELISA - Anti-mTOR (phospho S2481) antibody (ab45996)
    This antibody gave a positive result in ELISA against the immunizing peptide (Orange line, ab46195). It gave a negative result in ELISA against the non-modified equivalent peptide (Yellow line, ab61979). This indicates that it is specific for the modified peptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-mTOR (phospho S2481) antibody (ab45996)
    Immunocytochemistry/ Immunofluorescence - Anti-mTOR (phospho S2481) antibody (ab45996)
    ICC/IF image of ab45996 stained Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45996, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, HeLa and MCF7 cells fixed in 4% PFA at 1ug/ml and HeLa, Hek293, HepG2 and MCF7 cells fixed in 100% methanol at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

References (5)

Publishing research using ab45996? Please let us know so that we can cite the reference in this datasheet.

ab45996 has been referenced in 5 publications.

  • Xu J  et al. Co-enzyme Q10 protects primary chicken myocardial cells from heat stress by upregulating autophagy and suppressing the PI3K/AKT/mTOR pathway. Cell Stress Chaperones 24:1067-1078 (2019). PubMed: 31401771
  • Sato M  et al. a1A-Adrenoceptors activate mTOR signalling and glucose uptake in cardiomyocytes. Biochem Pharmacol 148:27-40 (2018). WB ; Rat . PubMed: 29175420
  • Qin A  et al. MicroRNA-126 regulates the induction and function of CD4(+) Foxp3(+) regulatory T cells through PI3K/AKT pathway. J Cell Mol Med 17:252-64 (2013). WB . PubMed: 23301798
  • Vazquez-Martin A  et al. Ser2481-autophosphorylated mTOR colocalizes with chromosomal passenger proteins during mammalian cell cytokinesis. Cell Cycle 11:4211-21 (2012). ICC/IF ; Human . PubMed: 23095638
  • Vazquez-Martin A  et al. Raptor, a positive regulatory subunit of mTOR complex 1, is a novel phosphoprotein of the rDNA transcription machinery in nucleoli and chromosomal nucleolus organizer regions (NORs). Cell Cycle 10:3140-52 (2011). ICC/IF ; Human . PubMed: 21900751

Images

  • ELISA - Anti-mTOR (phospho S2481) antibody (ab45996)
    ELISA - Anti-mTOR (phospho S2481) antibody (ab45996)
    This antibody gave a positive result in ELISA against the immunizing peptide (Orange line, ab46195). It gave a negative result in ELISA against the non-modified equivalent peptide (Yellow line, ab61979). This indicates that it is specific for the modified peptide.
  • Immunocytochemistry/ Immunofluorescence - Anti-mTOR (phospho S2481) antibody (ab45996)
    Immunocytochemistry/ Immunofluorescence - Anti-mTOR (phospho S2481) antibody (ab45996)
    ICC/IF image of ab45996 stained Hek293 cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45996, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in HepG2, HeLa and MCF7 cells fixed in 4% PFA at 1ug/ml and HeLa, Hek293, HepG2 and MCF7 cells fixed in 100% methanol at 1ug/ml. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

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